I referred to fact that intimin mutants were tested for adherence in cell lines and intestinal explants and in cattle but I cannot find assays in mice models.
Plant Cell-Based Intimin Vaccine Given Orally to Mice Primed with Intimin Reduces Time of Escherichia coli O157:H7 Shedding in Feces Nicole A. Judge, Hugh S. Mason and Alison D. O'Brien*
"Colonization of mice by E. coli O157:H7 is dependent on intimin.We determined that E. coli O157:H7 strain 86-24 Strr (35) reproducibly colonized female BALB/c mice at modest levels for approximately 10 days (mean, 6.4 to 13.4 days for unimmunized mice). Furthermore, in an experiment limited to 7 days, E. coli O157:H7 86-24 Strr colonized BALB/c mice a mean of 6.4 days, whereas the intimin-negative mutant 86-24 Strr eaeΔ10 showed a statistically significant reduction in the duration of colonization (mean, 3.2 days; P < 0.005; log-rank test)."
Alternatively, Citrobacter rodentium infection of mice has been used as model for EHEC and EPEC infections.
See: Citrobacter rodentium translocated intimin receptor (Tir) is an essential virulence factor needed for actin condensation, intestinal colonization and colonic hyperplasia in mice Deng W, Vallance BA, Li Y, Puente JL, Finlay BB.
Best,
Fabian
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