TdT vs. PAP for polyadenylation?

Hello, From my understanding, there are two ways to add polyA tails to RNA. 1) with polyA polymerase (PAP) and ATP to RNA or 2) with terminal transferase (TdT) plus dATP and ddATP to ssDNA...

18 October 2020 6,078 2 View

Nature of Racism?

I am writing a scholarly advocacy article - the question is: "What is the problem with using an historically specific example of racism as a template for understanding the multifaceted nature of...

29 May 2020 7,848 22 View

What effect size calculation to use for meta-analysis?

I am conducting a meta-analysis for the association between TNBC and BRCA1 and BRCA2. Can anybody help me as to what 2x2 frequency table to use- standardised mean difference, correlation...

05 March 2020 4,992 3 View

Have I got the settings right?

I want anyone to be able to download my paper "elderberry is antibacterial, antiviral etc" for free and easily. Have I got the settings right? With Covid-19 spreading I think elderberry (Sambucus...

02 March 2020 6,607 3 View

I'm looking for a measure of self-perceived attractiveness for a study I'm conducting at my university. Does anyone know of an existing measure?

I've looked around for a survey for this measure, but I can't find one anywhere. I originally was just using a universal 10-point rating, but my professor would like to see something more...

01 March 2020 6,529 3 View

GFP/RFP constitutively expressed from integrated transgene in E. coli?

It would be very useful to have tame strains of E. coli that are always fluorescent. So far the only ones I can find are from this work: Pinheiro, Bergquist et al. (2008), Applied Microbiology and...

09 February 2020 9,918 2 View

How many E.coli cells should I sort via FACS for RNA-seq?

Hello, I have been trying to find studies that have FACS sorted E.coli in bulk for RNA extraction and RNA-seq. Maybe I am bad at lit-searching, but I have been surprised to not find a clear...

14 November 2019 4,668 3 View

Preferred mRNA quantification from prokaryotes?

Hi, I am trying to put together a list of the methods used to determine how much mRNA you have after RNA extraction. My plan is to compare total RNA vs. mRNA amounts before and after rRNA...

25 April 2019 7,971 2 View

Why am I getting a lot of very small colonies when I do a transformation?

I'm currently working on my fourth try of transformation. Every time the plates have A LOT of tiny colonies (photo attached). Every once in a while there is a larger colony, but when I screen...

06 March 2019 6,823 8 View

What is the minimum volume of cerebrospinal fluid that I can use for ICP-MS?

I want to measure metal levels in individual mouse CSF samples. If some samples are only 7-10ul, are they still usable? Or do the samples need to be pooled? Also, any protocol for preparation of...

31 January 2019 3,320 4 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View