Dear Mangala,

      A number of years ago, Philippe Vandevivere and I developed some protocols to prepare bacteria for SEM, in order to preserve the exopolymers surrounding the cells, as well as the cells themselves. We tried different things, and finally ended up with some approaches that seemed to work very well. We used these approaches systemically afterwards, and always produced spectacular pictures, that looked tremendously better than with the traditional treatments.

      I append the manuscript here.

      Good luck.

      Philippe

Article Improved preservation of bacterial exopolymers for scanning ...

Thank you very much sir.I will use the method for sample preparation.