I have a SEM image of bacteria to segment that image using Snakes in matlab wanted some guidence referred matlab where I cudn't get any link which is suitable for image with many objects.
No not to post an example,but its a project .The individual objects in the image must be sepearted.
I wanted to try 'Snakes'.
This requires a lot of coding (matlab or openCV library ) . The pseudo -algo can be found + theory for deformable images
Feature Extraction and image processing by M Nixon and A Aguado
As John said it requires lotzzz of customization and localization for a specific object or region in the image
In general you can do image segmentation by using one method, but first you have to convert your SEM image to bmp image, it easier to do that. When you have bmp images try to make flowchart or pseudocode of your method to segment the images and convert it into matlab program.
I invite you (especially Harwikarya) to check out MIPAR (http://mipar.us), our new and very popular software. No need to make flowcharts or pseudocode. Our Recipes get recorded real-time and are completely non-destructive. Add, edit, remove steps whenever you wish and the rest auto-update. It is a true image processing playground. Thanks for taking a look!
Cheers,
John
As a lecturer when I teach my students I have to strengthen them how importance to understands the problem first, and next step they have to make their algoritmh by making flowchart or pseudocode, afterthat they can continu to convert these algorithm to any programming language. I do this because Informatics students must have the capability to do these. What you explain is ok for non informatics student, so they use snake as a tools, without needing to knows how snakes works.
I understand completely. As someone who has many years experience tackling many different problems - I absolutely appreciate how important creative algorithms and pseudocode can be to solving problems you never thought you could. What I am saying is that MIPAR has been shown to be very educational, since you can experiment with a near limitless combination of steps (algorithms) interactively, and efficiently, without necessarily having to know how to code, all while still understanding how each step works through our documentation. Thanks for your reply!
Thank you Harwikarya.
I really liked the way you have shown to get into the procedure.
I will try the that procedure.
John's discussion helped me to
I believe that morphological segmentation which combines morphological operations such as morphological gradient, with watershed flooding algorithm will give you good results.
Yes I have tried that and got some results.
But wanted to compare the results with snakes approach.As i have read in some articles and papers even snakes gives good results.
please refer to the link:
https://www.mathworks.com/matlabcentral/fileexchange/28149-snake---active-contour
Some inputs I got from the link. Applying for an image with overlapping and touching objects is really challenging one.
That book has pseudo code Mangala, also when it comes to region identification once done to separate them or identification of uniqueness , best use a logical representation or some threshold to differentiate the shape contour.
- Badges
- Science topic