Does anyone know how to precipitate silicon nanoparticles from acetone?

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Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I use different force field for protein and ligand?

Hello, Dear! Generally, I used CHARMM36 + cgenff force field for simulaiton. But,, due to certain parts of lignad, force field cannot be generated using Cgenff. Can I use different force field...

10 July 2024 9,513 3 View

Ways to mitigate stimulation electrodes coming off?

I have a cathode electrode (5 x 5cm) on the femoral triangle and an anode electrode (5 x 10cm) on the gluteal fold. We are doing repeated high-intensity cycling followed by repeated Wingates. We...

07 July 2024 833 1 View

How to solve Gaussian Convergence failure?

To whom it may concern, I'm working on optimizing the molecule using gaussian 16. However, it is suffering from the problem of convergence failure. I attempted optimization several times, but...

30 May 2024 6,373 3 View

Setting chlorine injection concentration??

I want to experiment with microbial control through chlorine disinfection. What I have on hand is 5% sodium hypochlorite solution. I want to set the chlorine injection concentration to 1, 3, 5,...

01 May 2024 3,741 0 View

What is the mechanism of ionization of transition metal?

can anyone give me advise about charge transfer transition of transition metal and rare earth element? and usually people see the FL and absorption, emission spectra of those materials in glass,...

08 March 2024 7,916 1 View

How to dissociate clumping cell?

H1781 cells are shown to tend to clump each other. H1781 is adherent cell and detached by using trypsin-EDTA. however, over-digestion using trypsin might provoke cell clumping. they seemed like...

03 March 2024 7,757 4 View

What is wrong with the mitochondria isolation protocol?

Hello I am currently trying to set up a new protocol. Our lab is planning to perform study about mitochondria, and we still couldn't set up a proper protocol. The kit we used is from abcam...

05 November 2023 9,681 0 View

Why have most research studies related to crack branching been conducted only on amorphous materials?

I've been studying the crack branching and dynamic fracture of brittle materials, and I just noticed that most of the experiments are conducted using amorphous materials like PMMA. is there...

26 October 2023 7,536 0 View

M-wave acquisition Matlab script?

I would like to acquire EMG, torque/angle (from isokinetic dynamometer), and stimulation data via Matlab. I have a chassis system that is connected to my computer with Matlab. When the stimulation...

18 July 2023 7,425 2 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Binder for ZnO nanoparticles in graphite paste electrode?

I have been using paraffin, but the deposited ZnO still detach from electrode. What is the best binder to modify graphite paste electrode with ZnO nanoparticles?

03 August 2024 4,624 3 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

In the presence of VERY high concentration (1000 ppm) of silicon, the adsorption isotherms of arsenic are showing anomalous trends, why so?

I conducted an adsorption experiment of arsenic on soil in the presence of different doses of silicon as competing ions to see the effect of silicon on arsenic adsorption and desorption. I took 5...

03 August 2024 6,500 3 View

Mossbauer spectroscopy Co0.6Mn0.4Fe2O4 MNPs?

Following curve related to Co0.6Mn0.4Fe2O4 Magnetic nanoparticles with extra phase a-Fe2O3. I will appreciate to help me to interpretation this spectroscopy, especially the blue line.

31 July 2024 9,140 2 View

What is the best method to detect the chemical composition of pollen ?

I have multiple small samples of grass pollen with anthers. I want to find a method to identify concentrations of proteins, lipids, and other various elements for these samples. any suggestions?

28 July 2024 5,331 1 View

Addition of EDTA during the synthesis of copper nanoparticles to prevent it from being oxidized?

I've attempted to use this method to synthesize copper nanoparticles. Copper nanoparticles can be synthesised using a variety of precursor materials. CuSO4, distilled water, NaOH, and EDTA are...

28 July 2024 8,027 3 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View