I have tried to solubilize rimonabant (CB1 inverse agonist) in DMSO (5% in saline or Ringer's Lacatate, 3 mg/mL) for intravenous injections in rats, but I haven't succeeded yet. The drug precipitates as soon as the first aqueous vehicle drop touches the mixture.
What are the other options?
Dear Filipe,
many thanks for posting this very interesting technical question on RG. I think that Victor already provided a rather comprehensive answer. I fully agree with him in that the SR141716 (rimonabant) should be first completely dissolved in the desired amount of DMSO gefore adding aqueous buffers. If the resulting mixture becomes turbid, it might be helpful to try sonification in an ultrasound bath for a few minutes.
The preparation of stock solutions of Rimonabant is described e.g. in the following potentially useful article:
DETERMINATION OF ENDOCANNABINOID RECEPTOR ANTAGONIST SR141716 (RIMONABANT) IN PLASMA BY LIQUID CHROMATOGRAPH TANDEM MASS SPECTROMETRY
Article Determination of endocannabinoid receptor antagonist SR14171...
Citation: "A stock solution (1.00 mg/ml) of SR141716 was prepared by weighing 1.12 mg of the compound into a 1.5 ml Eppendorf tube and dissolving it with 1.12 ml of DMSO."
Good luck with your work!
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