These cells derived from endometrial biopsies of women with regular menstrual cycle (proliferative phase). I read that I need to add estrogens (10nm), progesterone (100 nM) and cAMP (0,5 nM). In particular, I want to know the passage of the culture, how many cells I have to seed (confluence) and if the medium may contain FBS (10%) or not (1%). Finally, the time of the treatment. Thank you
Hi Maurizio, the cell number depends on the size of your culture dish/experimental set-up. There are several articles describing decidualization in vitro (for example Schutte et al. Fertility Sterility 2012: 97;997), with more experimental detail. With respect to the FBS; if you supplement with hormones you should always use stripped serum or Hyclone, otherwise you don't know the amount of hormones you are adding. Personally, I think 10% (stripped) FBS might be a bit high, because it strongly reduces the free hormone concentration in your culture.
I hope this helps, regards, Majorie
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