Maurizio Forte

3 Questions 4 Answers 0 Followers

Questions related from Maurizio Forte

Who can suggest a good protocol of IHC for detect beta amyloid in paraffin sections of rat brains?

There are other widely used marker for Alzheimer disease? I need to perform a colocalization? Fluorescent secondary antibodies or DAB (brains were fixed in Carnoy)? Nuclei staining? Any help on...

03 June 2016 7,592 1 View

Who can suggest a good plugin of image J for count cells after a migration assay with crystal violet staining?

I know that it would be better to stain cell nuclei with DAPI. However, when I take the images, I have a strong background of fluorescence, probably due to the use of cotton swab tfor remove cells...

13 March 2015 2,439 6 View

Does anyone know how to induce the in vitro decidualization of human endometrial stromal cells?

These cells derived from endometrial biopsies of women with regular menstrual cycle (proliferative phase). I read that I need to add estrogens (10nm), progesterone (100 nM) and cAMP (0,5 nM). In...

09 October 2014 4,574 1 View