I am trying to extract RNA from adipose mesenchymal stem cells seeded(6000cells) in the cardiac extracellular matrix hydrogel, but I have a problem getting reasonable OD number. Does anyone have any idea to help me? Would be much appreciated.
We are working on a thesis on simulating the effects of electromagnetic waves on the human body As part of our research, we want to examine the effects of mobile phone waves. Since Samsung has...
17 August 2019 5,045 3 View
I wonder if there is an association between various parts of an article DOI with information such as year of publication, issue and volume number of journal,For instance: 10.1016/j.asw.2013.09.004...
23 November 2018 244 7 View
rapid journal in biotechnology
02 April 2017 6,135 15 View
when I run the gel, GAPDH bands and my desire band stick together.Does anyone knows whats the problem?
29 January 2017 1,915 3 View
15 April 2016 4,459 13 View
Hi, for detection cellulose biosynthesis in E.coli nissle 1917 I used congored plate, but which condition( temperature and incubation time)is appropriate for just cellulose biosynthesis detection,...
26 February 2016 3,994 4 View
02 January 2016 1,392 5 View
I have used a questionnaire originally developed and validated in the UK with three constructs. In EFA am I allowed to use 3 as the base and in the extraction box instead of using eigenvalue of 1...
09 September 2015 9,294 8 View
Hi i have had 117 participants in a study and one of my variables has been their age. I used mean as the cut off score which resulted in 32 participants below the mean (mean=17) and the rest above...
01 January 1970 5,786 11 View
Since the amount of total RNA in the plasma is very low, about 50 ng, I wonder if I can do cDNA synthetise at the same time for my special RNA and housekeeping?
01 January 1970 1,379 5 View
I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...
02 March 2021 2,735 2 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.
01 March 2021 4,306 1 View
I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...
01 March 2021 7,675 3 View
Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...
01 March 2021 6,187 2 View
I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...
01 March 2021 8,079 2 View
The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.
28 February 2021 9,464 2 View
Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
28 February 2021 7,883 3 View
I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...
28 February 2021 7,330 4 View
28 February 2021 8,466 2 View