Can I use carbo-free blocking solution in place of normal goat serum?

I'm looking to try this in place of normal goat serum for a wisteria floribunda lectin stain. In my IF protocol, 10% of the final volume of my blocking solution is goat serum. If I were to go the...

07 February 2021 7,520 6 View

Can problems with blocking cause high background and weak antibody signal during immunofluorescence staining?

I'm working with 40um fresh frozen (non-perfused) sections of rat brain tissue and I'm staining for wisteria lection (immunofluorescence). After staining, my tissue has high background and a weak...

10 November 2020 3,620 7 View

Does anyone work with femoral and tibia radiography on rats or mices?

I would like to know what protocol followed for animals undergoing some treatment that induces some type of osteopenia / loss of bone density.

13 July 2019 1,731 2 View

What are the best research-based tools to teach pronunciation of L2 segments to second language learners?

Last year I attended an International Conference at the University of Utah about the teaching of pronunciation in second language learning and teaching. I notice that there was a general...

23 May 2018 5,599 2 View

Will thrombin aptamers bind immobilized/denatured thrombin?

I'm interested in modifying a polyacrylamide gel-based immunoassay to use aptamers in place of antibodies. In this assay, the proteins are covalently immobilized within the polyacrylamide gel via...

15 May 2018 5,490 2 View

How is your research likely to improve the approach to measuring performance quality?

I would be very interested in reading your publications related to measuring health and safety/ quality/ operations management performance - especially those related to recommendations for...

17 February 2017 7,501 2 View

Statistical Data: Experimental Design (teaching, how many students, control and normal group, definite conclusions)?

An innovative proposal, so say a new way of presenting contents or a small book with innovative ways of creating reasoning patterns: What do I need to have to prove to others, in a fully...

30 May 2016 9,386 3 View

Can you suggest a good assay to determine paracellular permeability in epithelial cells?

I am establishing a bovine oviductal epithelial cells culture using inserts of 0.4um pores and I am trying to get the cells polarized with this system. I would like to use the permeability assay...

16 January 2014 7,820 18 View

What is the role of the cerebellum in emotional processing and behavior?

Aims Neuroscience is requesting paper submissions for our April issue. Manuscripts will need to be received by February 28, 2014, and decisions on acceptance will be completed by March 28. Some...

19 December 2013 6,839 5 View

Is brain circuitry or brain chemicals the level that best explains psychopathology, including depression and schizophrenia?

The view that a chemical imbalance is the key to understanding and treating psychopathology has been dominant for years, but brain circuitry has become more popular as an explanation the past few...

01 December 2013 9,019 8 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

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02 March 2021 3,060 3 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View