Does anyone know how miR-21a induces the expression of total proteins?

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Huiwu Zhao

If you observe that over-expression of mir-21a could induce the total proteins, then mir-21a might promote cell growth. So one of targets of mir-21a could be a cell retarding gene, like PTEN, whatever.

Wq Yu

I used CD513B-1 as the vector of miR-21a.There is a GFP gene in CD513B-1,so that I can find the expression of the vector throug observing green light.

Eventually, you need a method to measure the mature miR-21a in your transfected cells.

Could I transfect the reserve RNA of miR-21a while detecting the target mRNA of its？