I would like to quantify the lesions on aorta. I want to take image of atherosclerotic plaques formation in aorta by using O red Oil Solution. After the staining, what will be the storage condition of aorta?
I also want to do the analysis of lesion formation in each part of aorta by oil red o staining. Can I do cryosectioning of same aorta and use it for further analysis? Do i need to stain the aortic parts again after the storage?
Thanks.
You can store the tissue cold in your sucrose solution prior to embedding in OCT, or embed it in the OCT and store frozed blocks. Performing the oil red O as a whole mount tissue first mau affect the texture of the tissue for cryosectioning. There are other histological stains that will work well after Oil red O. I do not know how antibody staing would work after oil red O because oil red O fluoresces, so immunohistochemistry may not work
- Badges
- Science topic
- Similar topics
- Engineering
- Petroleum Engineering
- Oil