In your opinion, which is the best software for reviewing Papers? Why?

How effective and helpful is it, really.

25 January 2021 6,988 73 View

Do chemical and physical properties influence on the size of a polymer particle?

I would like to know if the properties of a polymer impact on the size of the particle it forms. I'm working with different polyssacharides (alginic acid from different seaweed), which are...

29 December 2020 7,333 6 View

Asalam o alikum, Can anyone please help me in interpretation of XRD data?

Actually, I want to present the in table form (dcal , dexp, 2 thita, hkl) but don't know how to find these values from data.

28 December 2020 6,769 4 View

Is it need to test Cronbach's Alpha for extracted features from data?

As Cronbach's Alpha is usually used for test the reliability of measurements given by questionnaires, however, I wonder that whether I use this test for features extracted from behavioral data. I...

26 December 2020 9,602 2 View

Doping of r-GO?

if the percentage of doping Au into rGO is too small 1~10%, is it not enough to prevent restacking of r-GO in xrd results, rGO peak appeared at 22.2 and also the peaks of gold nanoparticles so...

21 December 2020 6,091 3 View

XRD peaks for gold nanoparticles ?

is the concentration of gold salt that were added will affect on the peaks appeared. i added a very little amount of gold salt to dope graphene oxide and not all the peaks that proved the...

17 December 2020 9,275 3 View

If someone has the pdf file of below mentioned reseach paper?

14 December 2020 5,935 3 View

Journal of Research in Humanities and Social Science. Is a real magazine?

Dear colleagues: I have received many emails from a magazine called ournal of Research in Humanities and Social Science. I have gone to the website of this journal, and I read "some" indexes but,...

13 December 2020 6,208 6 View

I saw sparkles in my samples?

i am trying to dope boron in graphene oxide , and after i take out my samples from the oven i put them in the microwave for 40 sec , and i saw sparkles . and fume also i couldn't understand why

16 November 2020 6,852 5 View

QUERY REGARDING acdS gene (ACC deaminase encoding gene) Amplification?

08 November 2020 3,249 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

No title

23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Does anyone have a working Guanidine Hydrochloride purification protocol for synthesized ssRNA?

I am trying to in vitro transcribe some fluorescein labeled ssRNA. And I want to purify the ssRNA from the free NTPs and the enzymes (RNA polymerase and DNase I). Due to the characteristic of the...

18 February 2021 4,392 4 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Agarose gel purification pre MinION sequencing?

Dear all, we performed an agarose gel purification with MinElute kit (QIAGEN) to extract bands between 3500 bp and 6000 bp for further sequencing with MinION. After the purification process,...

16 February 2021 5,178 1 View