Folic acid is conjugated to nanoparticles as a targeting agent to breast cancer cells. I am going to determine the conjugation efficiency and to detect conjugated folic acid in my solution.
You can check the success of immobilization by 13C CP/MAS NMR spectroscopy (it is a solid state NMR technique) or maybe also by (solid-state) ATR-FTIR spectroscopy. But it also depends on the material you use as the drug transporting material (maybe a triblock copolymer?). If the signals of the 13 atoms of the folic acid is overlaped by the 13C signals of the transporting material, then try 15N CP/MAS NMR. Good luck.
Maybe you can use fluorescence of folic acid. It´s not totally exact, but it´s a fist hint.
The shortest i can suggest is tritium labelled folic acid conjugation, and the perform scintigraphy to know the folate conjugation yield. Thanks
ftir, xrd, nmr
but the simplest method is the fluorescence spectroscopy of free folic acid that suspended in supernatant.
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