I have just started epilepsy research, and I am trying to examine the differences in epileptic activity in the mouse cortex under two different conditions. Since the typical waveform of epileptic potential that can be recorded by extracellular field potential (its polarity, frequency, duration, etc.) is not clear, it is difficult to distinguish between noise and epileptic potential.

Please let me know, if anyone has data on epileptic discharge in the mouse cortex in vitro.

I think that the epilepsy-like waveform in vitro can fluctuate depending on the multi factor, so I will describe my current conditions.

5 to 8 weeks ICR mice were decapitated after deep anesthesia with isoflurane. Brain removal takes 1 minute to 1 minute and 30 seconds. 400 μm brain slices were made on a vibratome under ice-cooling with a Colin-based dissection buffer. Slices were recovered in a 35 ° C holding chamber (self-made, submerged type) for 1 hour and maintained at room temperature until recording.

Recording was performed with ACSF heated to 32-34 ° C. The recording chamber is a submerged type, and the reflux rate is 7-8 ml / h. Tungsten electrodes were inserted into 2-3 layers of the cortex.

The composition of ACSF is as follows. all solutions were bubbled in carbogen gas (O2 95% + CO2 5%)

① normal ACSF ( to recovering chamber)

125mM NaCL, 3mM KCL, 1.25mM NAHPO4, 21mM NAHCO3, 2mM CaCL2, 1.5mM MgCl2, 10mM glucose

②modified ACSF (to rocording chamber)

120mM NaCL, 8mM KCL, 1.25mM NAHPO4, 21mM NAHCO3, 2mM CaCL2, 10mM glucose