Dear David,

Here are  a couple of papers which may find helpful.

1.Terrestrial orchid conservation in the age of extinction:

 Nigel D. Swarts and Kingsley W. Dixon

2. Asymbiotic seed germination, in vitro seedling development,and greenhouse acclimatization of the threatened terrestrial orchid Bletia purpurea

Daniela Dutra  Timothy R. Johnson ,Philip J. Kauth  Scott L. Stewart ,Michael E. Kane  Larry Richardson

Good luck!!

Hi David,

I've been in touch with Raheleh Dehgahi ([email protected]) in Malaysia over the past year on use of protocorm-like bodies (in Dendrobium sonia-28).  Could be of help to you.

Thanks,

I also found this review:

https://www.researchgate.net/publication/262850225_The_Application_of_Biotechnology_to_Orchids

I'll try different techniques. 

Article The Application of Biotechnology to Orchids

Please consult Prof. P. Tandon, Prof.S. Kumaria and Dr. M.C. Das of North-Eastern Hill University, Shillong, India.

Dear David,

I am from Hong Kong , South China. I once worked on Spiranthes sinensis and S. hongkongensis.We tried to germinate seeds of both species in lab and it was quite successful. We got plenty of seedlings within six months. 

Have you ever tried micropropogating your species with seeds rather than vegetative regeneration?

Good luck!

Dear Ai-Qun,

Thank you for your suggestion. I lead a restoration plan for a population at high risk in West Europe. I am in the prospective step where I have to find the most cost/time effective technique to increase the number of individual (from various spots in order to maintain genetic diversity) and for future transplantation. I believed that vegetative reproduction was the faster and most efficient technique. But I am happy to consider the 'natural' way if it is not too complicated with the medium and with the associated fungus to cultivate. As experienced scientist who know the flower genus what do you think?

You can write on my personal email if desired.

Many thanks again Ai-Qun