Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Can anyone suggest a commercial MAT kit to detect Toxoplasma gondii antibodies in animals?

18 January 2021 5,438 2 View

What is the difference between positivism and post positivism?

Your comments are being sought in regard of research methodology. Could you explain the meaning of postivist and positivism. How post positivism is different than the positivism?

26 December 2020 8,614 4 View

What is the purpose of internal control and extraction control in q PCR procedure?

Is extraction control also add to the qPCR mixture with the sample or does it run separately? Can anyone please explain their importance and how to use them in q PCR protocol? Thank you.!

23 November 2020 314 3 View

Can anyone explain the difference between recombinant proteins vs recombinant chimeric proteins?

Difference and advantages and disadvantages of using recombinant proteins versus chimeric proteins as antigens in serological tests

28 September 2020 5,307 3 View

How to find APC without open access choice in hybrid journals. ex springers ?

I was going through springer journal websites and only APC for open access is given. But I would like to know the cost of publishing without open access choice in hybrid journals. So how to find...

18 September 2020 5,861 3 View

Is there any possibility to detect both IgG and IgM antibodies in the same ELISA plate?

I am looking forward to developing an ELISA which can catch both anti-Toxoplasma IgG and IgM antibodies in Marsupials. Has anyone have any thoughts about detecting two different antibodies ( IgG...

24 July 2020 4,193 3 View

Can we calculate the average value of the particular sub-region of Raster TIFF image?

1) I have calculated various Drought Indices for all the 26 Mandals of the Warangal Region India. Can I calculate the average value of a particular drought index in any sub-region directly by...

19 April 2020 1,127 7 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View