i don't know much about your topic, but since calpain is activated through an increase of Ca2+ you could perhaps transfect your cells with the gene of an Ca2+ ion channel. Your gene could also have a compound specific promoter, which could be turned on/off by treating/not treating your cells with it. There should be some applications available, some ready to use vectors in which u can insert your gene of interest, respectively a gene coding for an Ca2+ ion channel.

don't know if this works, since the ion channels should also be activated somehow. I also heard about an application where ion channels are activated through light. Perhaps you can mix this applications together somehow.

good luck :-)

Hi.

in some Articles,I saw that induced ischemia activate calpain.some beta-antagonists same ISOPROTRENOL that in particular dose could induced ischemia that active the calpain in cardiomyocytes.

Good luck.

best regards.

Hi Soheil, 

thank you very much for your ideas. I`m going to check those publications. Ischemia should then be induced in the animals alive i think, am I right? 

My best.

A standard method which is widely used is increasing cytoplasmic calcium levels by using Thapsigargin to release the calcium out of the SR/ER or by bringing in calcium from the extracellular space using ionomycin or another Ionophore. We are using both substances regularly.