What am I doing wrong in ORR electrochemistry?

Hello I've recently reached the point in my work when I will need to conduct some electrochemical measurements, but unfortunately I have to start everything from scratch, because in our lab no one...

01 December 2020 3,447 3 View

Can I modify beginning of pelB/ompA tag as described?

Hello all, I am currently trying to modify my plasmids so that it has a sequence coding the pelB or ompA tag in front of the proteins. The problem is that in order to paste such a sequence into...

25 November 2020 8,386 3 View

Can I use an ELISA kit dedicated to plasma obtained with citrate or heparin if I used EDTA for this purpose?

Good morning all, My question is just like in title. I was using EDTA when collecting mouse blood, and most of ELISA kits are dedicated for plasma gained from blood collected with citrate or...

15 April 2019 2,737 2 View

Can I use my frozen samples to mark macrophages?

Hello all, my question is about using my frozen samples to detect macrophages. I have uterine cells, scratched with 40um strainer and frozen in -81 degrees in 90% FBS and 10% DMSO medium. They...

31 March 2019 1,837 3 View

What microscope setup do I need for comet assay?

10 September 2017 5,189 2 View

Could you give me a hand with my electrophoresis?

I've been performing RAPD-PCR for the yeast cultures I isolated from the Sauerkraut and everything was great. Subsequently I started to perform RAPD-PCR for the bacterial cultures I got from the...

08 August 2015 607 11 View

Is anyone using ELPHY Plus Ver. 5.0 to do the EBL?

27 January 2015 1,726 3 View

What are the best PCR-RAPD parameters to set when investigating Lactic Acid Bacteria?

I'm looking for best parameters for the PCR as well as best primers which I can use while investigating lactic acid bacteria. I was thinking of using M13 as a primer sequence, but I'd be glad if...

14 December 2014 6,658 3 View

Do you know any methods for learning from small data sets (and reasoning with such imperfect models)?

I am looking for solutions similar to leaky Noisy-OR gates in Bayesian networks, that allow for handling uncertainty caused by small amount of training data (see attached publication). The...

01 March 2014 3,282 5 View

How to download dataset?

I have to download dataset from software infrastructure repository (SIR) and I have created account on repository but I am not able to download plz guide.

25 February 2021 3,840 2 View

Repository for capture-seq raw reads?

I am a research tech in a wildlife genetics group and my PI and I are trying to figure out how/where to put raw reads in a repository. We have capture-seq NGS from Rapid Genomics. The post-doc who...

17 February 2021 7,575 2 View

How to use other researchers data set for our algorithm? should i cite or get permission?

Dear friends i have a query. i have to use a data which is a real time case study done by a canadian author. he has given data in repository. my question is when i use this data how should i cite...

01 February 2021 3,603 6 View

How to annotate list of gene names using Bash/R?

I have a list of gene names from deep sequencing from a constructed CRISPR knockout library. I was hoping to write a script to annotate all the gene names with a quick description of product...

27 January 2021 8,035 1 View

Submitting to arxiv before submitting to a journal?

Hello, everyone! Just wanted to know if I can first submit my paper to arxiv before I submit to a journal in elsevier. Hoping to find a clear answer. Thank you!

12 January 2021 5,266 3 View

Will increasing the number of synonyms in training set increase classifier efficiency?

Hello, If I increased the number of keywords (Features) of examples in training set pertaining to a particular class regarding to Sentiment Analysis Classification , will increase the efficiency...

11 January 2021 5,299 4 View

Recommendations for a microbial strain repository?

I'm looking for an open repository or culture collection to deposit some genetically engineered E. coli strains (from the papers below). The primary goal is to preserve those strains for the long...

11 January 2021 4,398 1 View

How important is the Golden ratio in the natural sciences?

The special Issue "Fibonacci and Lucas Numbers and the Golden Ratio in Physics and...

07 January 2021 7,408 6 View

How can we publish our articles in SSRN e-journal? Is it paid version or free? They are not responding in any letter?

I have a repository in SSRN library. I want to publish it in e-journal of Education in SSRN. The researchers who have contributed before may answer it.

15 December 2020 2,396 2 View

Which are the appropriate metrics can be used to calculate overall Precision, Recall and F-measure in different data samples? and when can use F1?

If we have three different domain of data (e.g. security, AI and sport) and we did 3 different case study or experiments (1 for each domain) and we estimate the Precision, Recall and F-measure for...

07 December 2020 9,833 3 View