Does anyone know a quick, non-invasive and cheap protocol to evaluate the immune response in small mammals ?

More Pauline Blandine Zablocki-Thomas's questions See All

How can women be responsive when they can make love for hours?

… with my present female lover … she and I spend anywhere from two hours to six hours in caressing, touching, cuddling, hugging, lip kissing, deep kissing and intimate conversation before,...

11 August 2024 4,521 0 View

Adhesion strength of coating?

How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...

10 August 2024 942 3 View

Why do men not accept that continually hassling for sex proves that they want it more than their partner?

Your partner’s not there to service you, it’s not their job to keep you sexually satisfied. You’re together because you love each other and want to make each other happy. Constantly hassling them...

08 August 2024 1,491 0 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

Polymer wear calculation?

What is the method for analytically calculating the wear and service life of polymer-polymer sliding pairs?

04 August 2024 1,078 1 View

Why do women not understand that men are aroused by physical contact?

Women often complain that their husbands never touch them unless they want sex. (Michele Weiner-Davis)

02 August 2024 7,778 2 View

Why do women usually need more persuading than men do to have sex with a new lover?

Women need to feel a degree of sexual intimacy before sex becomes desirable… For women, intimacy sometimes results in sex; for men, sex sometimes results in intimacy. (Marina Muratore)

31 July 2024 8,860 0 View

Why do men and women confuse platonic love and sex?

Women associate affection with love. … Men associate affection much more directly with sex. … Men see affection of any kind as a sexual invitation. Many women find this bewildering. (Kramer &...

30 July 2024 9,498 2 View

Why is it not common to see similar pairs in polymer sliding pairs?

If the pairs are similar, will it reduce the adhesion due to electric charges?

29 July 2024 3,185 2 View

Why do women use fantasy to achieve arousal alone?

Women also often find it easier to fantasise when self-pleasuring than in sex with a partner. The immediacy of someone else’s needs actually inhibits the expression and satisfaction of their own....

26 July 2024 8,351 2 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How long will macrophages remain in the M1 state after treatment with 100 ng/mL of LPS?

In my experiment, I treated RAW macrophages with 100 ng/mL of LPS. After 24 hours, I collected the supernatant and replaced it with fresh media. I then quantified the cytokine levels of TNF and...

01 August 2024 476 2 View

Learning in Animals with Unlimited versus Limited Neurogenesis?

“Yerkes (1912) trained an earthworm to choose one arm of a T-maze, using electric shock as punishment for error and the moist burrow as reward for correct choice. The habit was acquired in twenty...

31 July 2024 8,809 0 View

What are the steps of formulating a herbal extract into a sheep/goat drench?

I am working on natural remedies for de-worming in small ruminant livestocks. Part of my project involves formulation of drench-type product, which I have not much experience in. My experience is...

30 July 2024 9,806 1 View

Can I proceed with Response Surface Methodology (RSM) with only one factor for further optimization?

I went for Plackett Burman Design for selecting the optimization factors of growth conditions for pesticide degradation. I started with 7 parameters. But, after the experiments and analysis, only...

29 July 2024 5,015 1 View

(treating astrocytes with LPS) Has anyone observed the recovery of astrocytes from LPS injury, a few hours after the treatment?

or can you recommend articles showing the differential effect of LPS in a time dependant manner?

28 July 2024 2,547 1 View

Are the apoptotic cells is positive for γH2AX ?

It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...

28 July 2024 7,983 2 View

Has the Affordable Connectivity Program Helped You/Your Community? And Would Bulk Tablets on a Similar Program Help Your Business?

Do You Believe VP Harris Would Show Empathy to Tech Companies in Her Administration as POTUS? I ask in light of the fact that, the ACP was not re-funded this round. So many and businesses need...

24 July 2024 4,732 3 View