Help me conduct a neuroscience experiment for my master's thesis?

22 January 2021 9,994 5 View

Continuous glucose monitoring in animals ?

Is continuous glucose monitoring, comparable to what is existing in humans, available for mice and/or rats without large surgery ? thank you !

21 September 2020 5,950 2 View

How to converge hyperelastic modeling with large rotations in Abaqus?

Hi, My problem is similar to simulate fold soks in 3D model with 2D elements (shell). I would like to use Membrane element but it is not possible for hyperelastic material in abaqus. I use S3 (or...

17 March 2020 795 5 View

How to properly calculate the mutational rate in bacteria?

Hello everyone, I hope y'all can answer a simple but problematic question. I have been doing mutation accumulation experiment in 3 parallel lineages in bacteria and I now want to calculate the...

05 December 2019 5,648 4 View

Does anyone know how to implement restricted permutations for multivariate analysis of split split plot experiments in R?

02 April 2019 4,257 1 View

Is there any effective transfection protocol for cells cultured in 3D hydrogels?

I would like to transfect micro-RNA mimics in 3D models. Lipofectamine RNAimax works well in monolayer cultures but I couldn't find convincing evidence it could work sufficiently well in 3D.

27 November 2018 4,524 3 View

How to design a low ecological footprint electric machine?

To my knowledge, electric machines (motors and generators), apart from their electronics, are made of at least two or more of these materials: Electric steel (stator and rotor iron...

08 November 2018 1,783 9 View

Quickly pellet floating fatty cells in culture plate?

Hi, I have fatty floating cells in culture medium and I need to simply/quickly pellet them without degrading cells nor removing themfrom the plate. Any idea ? thanks

21 March 2018 4,569 3 View

Suggestions for an undergraduate research project concerning Bryophytes and Plant Soil Feedback?

I'm planning my Undergraduate Research Project and am very interested in the field of Bryology (I'm majoring in Botany), and Plant Soil Feedback, but am having a tough time coming up with a...

16 January 2018 9,890 4 View

Help with NCBI table2asn

Does anyone have experience with the table2asn tool of NCBI? It is used for submitting annotations of genomes using GFF files. It can be found at this location:...

23 May 2017 1,696 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View