What order is the change in the shift or intensity of a XRD pattern when there is an impurity in the crystal lattice?

I doped a fluoride perovskite LiMgF3 with different concentrations of Cr (0.25%mol and 0.5%mol). The XRD pattern of LiMgF3 shows the highest peak at 38.9847, but LiMgF3:Cr 0.25%mol shows this peak...

06 July 2020 5,630 4 View

Does perampanel belong to NTI drugs?

29 July 2019 7,372 1 View

Is there any simple way to count how many synonymous and nonsynonymous SNPs have in a VCF file?

I have 30 isolates, of these I need to know from each how many synonyms and non-synonyms SNPs I have. Is there a faster way to count without having to be one by one? These isolates had a high...

16 July 2019 4,784 1 View

Should I open Sigma Aldrich MS liquid medium inside the laminar flow cabinet?

I just bought MS liquid medium, micro and macronutrient separately. I wont use the whole medium on a single time, so I must open it several times. My question is, should I manipulate it inside the...

06 May 2019 375 11 View

Is there any way to re-embbeding a plant tissue that not infiltrated?

The tissue was embbeded in Spurr Resin. The infiltration process was 1:3, 1:2, 1:1, 2:1, Pure (Resin x Acetone). When I went to make the cut, I coudn't cut nothing. Is there any protocol to...

24 May 2018 7,829 2 View

Does valproate belong to narrow therapeutic index (NTI-drugs)? Is there a list for NTIs in Europe?

thank you for information in advance: Is there a list for NTIs in Europe?

13 May 2018 4,744 6 View

Hello. What is the point of zero charge of silver nanoparticles?

I synthesise silver nanoparticles using orange peel extract.

14 March 2018 9,226 3 View

How can I immobilize an enzyme in polypropylene powder?

05 October 2017 9,582 7 View

What solvent should I use to encourage the precipitation of silver nanoparticle? what volume ratio should I use?

I synthesise silver nanoparticles using orange peel extract

17 September 2017 9,693 3 View

Multi nominal logistic regression

12 February 2017 4,048 6 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View