Does anyone know a good efficient protocol to isolate nuclei from Drosophila larvae nuclei to be used later in native MNase-ChIP as well as 3C assay?

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Nurul Hidayah Ahmad Suhaimi

May I know which organ you want to isolate the nuclei?

Tamer Ali

the whole larvae...

Ivette Pacheco-Leyva

I have this protocol that I've been using for extracting nuclei from cultured cells. doi:10.1101/pdb.prot4767

It works perfectly for me, although I do not have any experience on extracting nuclei from larvae or tissues, etc. Probably it might work for you, if you decide to try it, and if you need help, do not hesitate to contact me. Good luck.

thank you Ivette, Cell culture protocols can not be applied on larvae .. i checked that myself...do you have any other ideas