Nurul Hidayah Ahmad Suhaimi

2 Questions 4 Answers 0 Followers

Questions related from Nurul Hidayah Ahmad Suhaimi

Is it a possible and reliable result, if the gene expression by qPCR shows 2 peaks in one sample?

I have undergone the expression by qpcr, and I have used one primer to test for several templates. Somehow, few and almost all of the samples showed 2 (double) peaks in the melt curve of the...

07 July 2014 2,964 9 View

Why do many peaks result in the melting curve of RT-qPCR?

I had done a thermal gradient for my real time PCR. Somehow, the result shows many peaks in the melting curve.

01 January 2014 9,214 9 View