Statistical Analysis for multiple measures in same person?

For my master thesis I am conducting a research into Myasthenia Gravis (MG). The question I am trying to answer is: Does the AChR-concentration in a blood sample correlate with clinical symptoms...

25 November 2020 3,217 3 View

What is an alternative test, if the assumptions for ANOVA and Kruskal Wallis test are not met?

Hello everyone, I ran in some issues, when i wanted to test my data. My data consists of 7 monkeys with the frequencies of stress behaviour, self grooming (SG) and stereotypical behaviour (ST)....

30 January 2019 1,277 8 View

What is an alternative for the chi squared test?

Hey, I am having trouble with finding the right statistical test for my data. I have a frequency of two types HMA and LMA and their morphologies. First i thought that the chi squared test was the...

01 November 2018 1,340 13 View

How to analyse a Bimodal response variable?

Hi, I am working on my master thesis, I want to test if using different kind of messages results in a higher likelihood to accept online cookies. To do this I have a model with two dependent...

20 June 2018 369 4 View

Does anyone have experience with the synthesis of palmitate-BSA and the subsequent uptake by the HuH7 cell line?

12 February 2017 4,538 3 View

I am searching for the average dietary/nutritional intake of the Mauritian people. Must include macro and micro nutrients. Somebody help me please?

For my project in Mauritius I study the causes of their high (growing) prevalence in diabetes. In order to sort out all the causes and give them value we also need to look at their average...

15 November 2016 3,489 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View