Does anyone know a CD127 antibody that crossreacts with sheep for flow cytometry?

More Daan Ophelders's questions See All

Is there any research on the reception and dissemination of the concept of 'ideology' in France from circa 1830 to 1950?

I am conducting research on the evolution and dissemination of the concept of ideology. I have observed that several works, aiming to provide an overview of its history, contain notable gaps. For...

08 January 2024 8,617 0 View

How to i merge duplicate?

I found out I have to Reasearchgate accounts each with its own list of publications. How do I merge them?

18 October 2023 4,615 4 View

What would be the best text by Gramsci to use with students who have never read his work?

I struggle to find a text by Gramsci that is a good introduction to his philosophy for students that are unfamiliar with his work. Preferably the text should be on hegemony and historical blocs.

19 September 2022 4,102 3 View

Statistical Analysis for multiple measures in same person?

For my master thesis I am conducting a research into Myasthenia Gravis (MG). The question I am trying to answer is: Does the AChR-concentration in a blood sample correlate with clinical symptoms...

25 November 2020 3,399 3 View

About HLA typing; how to determine specific HLA supertypes from DNA?

I would like to identify specific HLA supertype (e.g. HLA-B44) using whole blood. 1. What would be the best approach? 2. What are the alternatives of determining all associated HLA alleles (in...

26 October 2020 7,285 5 View

Which fungus has the most glycoside hydrolase genes?

I am searching through literature to find which fungus has the most glycoside hydrolase (GH) genes. My current winner is Fusarium oxysporum with 396 GH genes reported, but the source is from 2013...

13 January 2020 9,024 0 View

What is an alternative test, if the assumptions for ANOVA and Kruskal Wallis test are not met?

Hello everyone, I ran in some issues, when i wanted to test my data. My data consists of 7 monkeys with the frequencies of stress behaviour, self grooming (SG) and stereotypical behaviour (ST)....

30 January 2019 1,442 8 View

What is an alternative for the chi squared test?

Hey, I am having trouble with finding the right statistical test for my data. I have a frequency of two types HMA and LMA and their morphologies. First i thought that the chi squared test was the...

01 November 2018 1,506 13 View

How to analyse a Bimodal response variable?

Hi, I am working on my master thesis, I want to test if using different kind of messages results in a higher likelihood to accept online cookies. To do this I have a model with two dependent...

20 June 2018 581 4 View

How to report an OR when continuous data are log-transformed for logistic regression?

E.g. - "The OR of Zn on a logarithmic scale is ... per mcg/l" ? or - "For every change of factor 10 increase in Zn concentration, the OR is …per mcg/l ? or ... ?

13 May 2018 6,788 2 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Problem with Two-phase simulation?

Hello; I am conducting a CFD study on two-phase flow in a separator, utilizing refrigerant as the working fluid. For this study, I have implemented pressure inlet and pressure outlet boundary...

25 July 2024 2,309 5 View

Flow through curved domains?

Hi everyone, I am working on a curved domain in which a ship is situated in the middle (geometry is given below). In my understanding the general fluid flow is parallel to the x axis from inlet to...

25 July 2024 9,058 4 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

Saleh Alkarim

Hello

Check on biocompare , R&D system , thermofisher , novusbio , biolegend ,

Good Luck

Mousa Mohammadnia-Afrouzi

Dear Daan

You can check its reactivity in company website.

Jean-Jacques Pin

It is quite possible that the R34.34 clone directed against IL7R (CD127), which recognizes the human form, but also the canine form, may cross react with the sheep. To be tested. this clone is marketed in Europe by Enzo Life