I'd like to check the identity/integrity/validity of the plasmid with the alternative names above. The size is too large for a simple gel, so restriction fragments should rather be inspected, but for that I'd need to know the sequence to see if the pattern is correct.
I could not find the sequence in NCBI - Addgene - DSM - Stanford - etc... :(
So we'll blindly use it and if it works, then fine. :)
Please check the catologue of DSMZ by contacting :
Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures
Inhoffenstraße 7B
38124 Braunschweig
GERMANY
Phone: +49 (0) 531-2616-0
Fax: +49 (0) 531-2616-418
E-mail: contact @ dsmz.de
Here's a PDF of the original paper describing the construction of pRK2013, not as nice as having the actual sequence but hopefully it helps.
(If I'm reading this right, digesting with PstI and HindIII should produce a fragment ~7.2 kb.)
Article Figurski, D.H. & Helinski, D.R. Replication of an origin-con...
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