Let me know whether the DOI issued by the research gate will be accepted by the anna university?

As per the rules and regulation DOI is needed and paper has to be opened in online by the anna university rules let me know whether the published article in some journal and the DOI issued by...

27 November 2020 7,540 2 View

Can anyone suggest a way to overcome recombination when amplifying a pcDNA3 plasmid containing human SCN9A CDS insert?

I need to amplify a pcDNA3 plasmid containing human SCN9A CDS insert (pcDNA3-SCN9A construct). The CDS is around 6 kb, and the total construct length is approximately 11.2 kb, which is a bit...

07 June 2020 2,134 2 View

How to develop MCF7 mouse xenografts?

Hello everyone, I have two questions, please shed some light on this. I am new to in vivo studies and some may find my questions are trivial. TIA. My first question is, I wanted to develop the...

13 October 2019 1,208 2 View

Can anybodu suggest a good journal to publish the work on "STEEL TRUSS OPTIMIZATION USING GENETIC ALGORITHMS AND 2D TRUSS ANALYSIS PROGRAM IN MATLAB"?

We have done a work on "STEEL TRUSS OPTIMIZATION USING GENETIC ALGORITHMS AND 2D TRUSS ANALYSIS PROGRAM IN MATLAB". We would like to publish it. So we need good journal to publish the work.

15 March 2018 6,442 2 View

Looking for opensource tool for ReaxFF simulation. If there something other than paid software ADF please reply ?

Looking for opensource tool for ReaxFF simulation. If there is something other than paid software ADF please reply ?

24 January 2018 6,596 5 View

Can anybody explain how to find the permeability of pervious concrete experimentally?

We are working on a project for studying the characteristics of pervious concrete. We are planning for fabrication of a model for finding the permeability of pervious concrete.

13 November 2017 3,567 7 View

Is it necessary to synthesis second strand cDNA to amplify the gene of interest?

Dear All, I have synthesised cDNA first strand using SuperScript III (Invitrogen) and would like to amplify my gene of interest for overexpression studies? Is it necessary to synthesis second...

08 November 2017 5,895 11 View

How to clone a 5 kb insert to a vector successfully?

Hello, everyone. I'm trying to subclone a 5 kb gene to a vector (approx. 5 kb). Initially, I designed the primers which have restriction enzymes overhang (XhoI and XbaI). I amplified my gene using...

27 August 2017 4,722 4 View

Does accumulation of Enzyme- substrate complex possible inside a cell? Is there any experimental evidence which confirms their accumulation?

I am a systems biologist trying to study cellular cross talk. Some computational papers have mentioned about the effect of enzyme substrate complex on the cellular cross talk. So, my question is...

18 December 2016 7,172 3 View

No title

25 July 2016 7,622 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Compact antenna for the frequency range 1 MHz-5 MHz?

Hello everybody! I have a problem with developing a compact antenna for the 1MHz-5MHz frequency range. Tell me what type of antenna can be used and what books and articles to read to perform this...

27 February 2021 7,710 4 View

Where i will get the Desmodium and Napier grass seeds?

26 February 2021 975 4 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

What does it require to be ready for e-Commerce in developing countries?

Most developing countries are not ready for e-Commerce? What dimensions and indicators are there to measure e-commerce readiness of a nation? Please share framework or model and evaluation...

22 February 2021 8,132 6 View

How to use Remote sensing and GIS applications for mapping the available medicinal plants within the locality/municipality?

Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis...

21 February 2021 374 5 View

Can I perform two way ANOVA in case of some missing groups?

I had control cells and performer ELISA analyses group (time 0). I stimulated cells with substance A and B and cultivated for 1, 2 and 3 hours. On each cells I performed ELISA. I have results...

17 February 2021 8,933 4 View

Any recommendations for methods to analyze elephant space-use (using GPS) and correlating this with changes in NDVI?

For my Masters I am looking into elephant space-use and its effect on vegetation change. I have elephant GPS collar data and was going to use Google Earth Engine to obtain NDVI values. I would...

16 February 2021 7,097 3 View

Reliable and Valid Instrument for Causes of Medical Errors?

Is there any questionnaire/survey that measures the causes of medical errors? Example: physician negligence vs. systemic issues. Looking for a reliable and validated instrument. Thank you!

15 February 2021 2,874 4 View