Does anyone have the B104 rat neuroblastoma cell line that they could send to Eastern Canada?

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About exosome isolation?

Hi, All, I am relatively new for exosome isolation. I used an izon column 70nm to isolate exosomes from differentiated neural cells, but the concentrations were pretty low after NTA analysis (I...

01 July 2024 2,558 0 View

Effective of teacher personality on children participation in activities ?

20 May 2024 7,434 0 View

Academic Term Papers and Seminar papers can i publish them?

I have lots of Academic Term Papers and Seminar papers which under go thorough literature reviews and are well reference but do not follow journal article guidelines. Can i publish them? Saba'atu

11 April 2024 1,729 2 View

What software can I use to compare two annotated genomes downloaded from NCBI database?

Which software to compare two genomes

09 April 2024 4,571 2 View

My son Rowan is studying 'Neuropsychology - Influence of Meditation on Adult Autistic Individuals' for his Research Project. Can anyone answer?

I have purchased a commercial grade EEG machine, for a reasonable price, in order to perform a study for my project. How familiar are you with less sophisticated EEG equipment? To what extent...

15 March 2024 9,449 5 View

US-based labs for hair cortisol analysis?

We are looking for labs based in the US that assess cortisol in human hair samples. We used to send samples to Mark Laudenslager's lab at the University of Colorado for analysis. We saw another...

29 February 2024 3,225 0 View

Using multiple role theory to examines leadership roles of school heads assigned in two schools?

i need to know what leadership roles used by the school heads who are assigned in two schools by using multiple role theory

12 February 2024 8,346 2 View

Please can someone show me in detail how I can calculate MAAT, MAGST and TTOP using live data from three different sites?

14 January 2024 1,616 0 View

Regarding expiration time for urine samples for drug screens is it always 2 hours or 4 if refrigerated ?

i am finding that drug court officers abiding by SAMSHA best practices protocol set up decades ago, have not been educated in interpreting results (they arent medical students) They look for the...

10 January 2024 440 2 View

Vector backbone alone eliciting a response in plant pathogen defense?

My colleague is trying to study the effects of a protein on pathogen inhibition in N. benthamiana. He is expressing it in either a pSITE vector or pCAMBIA1302 that had the GFP gene removed. They...

07 January 2024 3,413 1 View

Which cell line will be the reference for human neuroblastoma?

We are implementing a grant related to the study of supertargets in secondary cultures of human neuroblastoma cells - IMR-32, Kelly, SH-SY5Y, SK-N-AS etc. Comparing gene expression between cell...

16 April 2024 7,706 2 View

Protein extraction from SH-SY5Y neuroblastoma cells and differentiated neurons?

I am working with SH-SY5Y cells and differentiating them into neurons using retinoic acid. I need to extract protein from them in order to conduct a western blot, however, I am having trouble...

31 January 2024 5,056 3 View

Can anyone identify what type of cell culture contamination this is?

Hello, Our lab is struggling with contamination despite taking every precaution necessary to maintain a sterile environment. The images shown are mature iPSC-derived neurons, but the...

03 October 2023 9,110 4 View

Any retinoic acid protocols for differentiating IMR32s?

Hi all, I am trying to differentiate my IMR32 neuroblastoma cells using retinoic acid or a similar treatment, and was wondering if anyone had any protocols you've had success with that you would...

10 August 2023 8,042 0 View

Contamination in sk-n-sh neuroblastoma cell culture?

Hi, I’m trying to culture sk-n-sh neuroblastoma cells. After 8th day of the culture cell morphology disappears and the accumulated structure appears (Please see the photographs). Could anyone make...

29 July 2023 4,952 0 View

¿What abtibodies can I use to evaluate oxidative stress in a cell line Using immunofluorescence ?

Hi, I want to evaluate oxidative stress in a neuroblastoma cell line, I know about SOD1, but I think it's not enough, so I was investigating and I found 4-hydroxynonenal, I have this in my lab...

27 June 2023 9,084 3 View

Which are the best conditions to transfect SK-N-SH with Nucleofector (Amaxa)?

We want to transfect SK-N-SH cells with Nucleofector (Amaxa), but our device don´t include the proper program to do it (X-005 or X-05). Does anybody know which are the settings corresponding to...

15 March 2023 499 3 View

How long would it take to differentiate SHSY5Y cells with retinoic acid?

Hi all, I am trying to differentiate SHSY5Y human neuroblastoma cells into neurons with the retinoic acid protocol. I add 10uM retinoic acid into 3% FBS containing DMEM for three days, and...

21 February 2023 8,474 4 View

Looking for human neuroblastoma cell lines from different stages of disease?

Hello everyone. I am looking for human neuroblastoma cell lines from different stages of disease. To clarify, i am looking for NB cell lines that are representing patients with different stages of...

25 January 2023 5,054 0 View

What is the most common normal neuronal cell lines ?

Dear all! now im working on my thesis (Anti Cancer Effect of Multi walled carbon Nanotubes ). im using SH-SY5Y neuroblastoma cell line and i need a normal neuronal cell line in order to test the...

21 December 2022 4,604 2 View