OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Quality of antibodies from Creative Biolabs?

It seems Creative Biolabs has everything and so many versions of antibodies not owned or sold by any other company. How reliable are their products? I am looking for a clone where potentially...

08 February 2021 2,167 3 View

Benefit of CTCF (corrected total cell fluorescence)?

03 February 2021 6,302 2 View

Why do the absorbance values of different concentrations of ascorbic acid solutions mixed with DPPH free radicals stay constant?

26 January 2021 458 14 View

Interpreting regression findings prior to inclusion of control variable?

I'm looking for advice on interpreting a statistical relationship - it's for a paper I am reviewing. (Variable names have been changed). It was found that: [IV anxiety + control variables y and...

06 January 2021 558 3 View

Can someone help me with an extraction protocol?

Hi all, Does anyone have a protocol for extracting DNA from agarose that is only 120 bp without a kit? The protocols I have tried are for much larger fragments and it is just not working. Thank you!

10 September 2020 1,291 2 View

Advice for getting dermatopathology tissue to stick to slides during immunostaining?

I work in a veterinary pathology lab and we do immunohistochemistry staining by hand on all types of tissues that are FFPE. We have particular difficulty getting dermatopathology (skin) samples to...

26 August 2020 792 1 View

Validation of experimental results?

Is it neccessary to perfrom validation of process model established using a box-benkhen experimental design? How "big mistake" will it be if I finish the experiment after performing the runs...

24 August 2020 6,917 3 View

Any reliable protocols for tissue detection of DNA damage with IHC or IF?

Dear all, Hope as a community your are doing fine. I have a question regarding antibodies against gH2AX, XRCC1 and p53bp1 that have worked for tissue detection for either IHC or IF, using...

08 June 2020 1,461 3 View

Where can I find the parameters of Miltenyi gentleMACS protocols?

I'm planning to use a Miltenyi brain tumour dissociation kit, but without the gentleMACS machine. Does anyone know where I can find the parameters of the machine's programs/protocols?...

16 December 2019 6,150 1 View

The best siRNA transfection protocol for SK-N-AS cells?

Hello, I am looking for protocols which would be effective in the neuroblastoma cell line SK-N-AS? Thank you.

12 September 2020 8,769 3 View

What is the pathophysiology of the neuronal tumors?

Different types of neuronal tumors occur in children such as neurocytoma and neuroblastoma. I look for any evidence and details about the mechanism by which a neuron can develop a tumor.

26 August 2020 1,403 2 View

Possible explanation of extremely high cell death in neuroblastoma SH-SY5Y under tert-butyl hydroperoxide incubation?

Dear collegues, Recently I've faced a strange problem using tert-butyl hydroperoxide for induction of oxidative stress and cell death. I've worked with neuroblastoma SH-SY5Y cell line. My aim was...

12 August 2020 8,067 1 View

I would like to look at CD34 subsets on BM from neuroblastoma patients, by Flow. Namely CD34/CD133, CD34/CD90 and CD41. Any thoughts on best clones?

I want to look at CD34 subsets in neuroblastoma pts, (bone marrow) before and after treatment. CD34/117, CD34/38, CD34/133, CD34/90 and CD34/41. I have clones for CD34, CD38 and CD117 but I cannot...

25 June 2020 4,146 2 View

Reasons for Covid-19 false positives in pediatric cancer?

Hello, Any idea why false positives appear in children with cancer (neuroblastoma, osteosarcoma, etc) under chemotherapy? Rapid tests result positive but PCR results negative. Thanks,

17 June 2020 3,277 3 View

Serum starvation before transfection and cell doubling time?

Hi, everyone I have a simple question about timespan of serum starvation and transfection. Recently I work on U-2OS cells which have doubling time around 20 hours, and before I transfect U-2OS...

15 March 2020 3,293 4 View

What are the possible reasons for xCELLigence impedance being negative?

We are trying to measure the migration of neuroblastoma Neuro2a cells using xCELLingence system with CIM-16 plates. The plates have two chambers: the upper contains cells, the lower contains...

31 December 2019 9,055 2 View

Hello, I am wondering which of these cell lines (WI38, HEK293) could be used as control for analysis on osteosarcoma and neuroblastoma cell lines?

I am researching lncRNAs in pediatric cancers - neuroblastoma and osteosarcoma, and I am unsure of a control cell line to use. Ideally, i was hoping to verify differential expression among the...

11 December 2019 8,047 2 View

GPR39 Antibody in western blot?

Hello, I am studying GPR39 in differentiated neuroblastoma cells. GPR39 has a molecular weight of 51kDa, I am getting a week band on 51 kDa but a stronger band on 70 kDa. Any ideas on what happened?

11 December 2019 3,155 1 View

What are the ideal conditions and medium for culturing neuroblastoma SH-SY5Y cell line?

I am working on the SH-SY5Y cell line. But facing problems in culturing as my cell line doesn't grow. Kindly provide me details of media and condition with the procedure. References will be...

10 November 2019 269 1 View