Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

Cyclooctatraene damaged by TFA?

16 February 2021 4,747 3 View

Inexpensive eye tracking hardware + software solution?

Hello, I am starting a project that will require an eye tracking device. I've seen eye tracking hardware + software solutions that are $10,000+ so I am hoping to find one that is significantly...

18 January 2021 8,121 12 View

What materials besides gold can be infused into window panes?

Hello, Driving around, I have noticed a lot of office buildings where the glass panes have been infused with gold. The thin layer of gold reflects the sun and keeps the office at a comfortable,...

06 January 2021 976 4 View

Why build a Leaning Tower of Lire?

What are the lessons to be learned from building a leaning tower of Lire?

03 January 2021 7,364 4 View

What are some of the applications of transparent wood?

Are there any particular uses for transparent wood other than visual appeal? The transparent wood is really cool to see, but what are the practical uses? Please provide examples. Happy New Year!

31 December 2020 2,479 4 View

Can light waves induce electricity through induction?

Can light waves induce electricity? I am unclear on this matter. Thank you.

30 December 2020 7,974 3 View

What are the benefits of applying Blockchain within any industry?

Is blockchain only for cryptocurrency? Thanks in advance.

28 November 2020 5,086 11 View

Is my Chi-square test valid?

18 November 2020 3,033 2 View

Confused primer dimer or something else?

Hi everyone, i recently did a qPCR to check the best annealing temperature for my primers. So i ran two of my primers on annealing temperature 59C with a melting curve. The melting curve shows...

17 November 2020 1,913 5 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View

Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software?

Hi every one Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software? Biogas fuel is not available in the options thanks

23 February 2021 6,518 3 View

How can I improve the concentration of banana fruits extracted RNA?

Currently, I'm working with banana fruits. I've tried to do RNA extractions with different methods, and I've gotten total RNA without contamination, but I couldn't get a high concentration of...

22 February 2021 6,126 2 View

How the standard hydrogen electrode value is convertible into reversible hydrogen electrode?

As much I remember +0.0592*pH, as I am working on CatMAP, so my voltage values are in SHE, not RHE.

18 February 2021 1,685 3 View

Does calculating the interaction index between a drug and a food matrix make sense?

Dear all, I'm conducting the revisions of some of my manuscript, with is the impact of the combination of Aspirin and a fruit juice in colorectal cancer cells. Two of the reviewers have asked to...

16 February 2021 3,141 3 View

Can explain this diagram to me, is this the average consumption curve for one day or the total consumption of days in a month summed up in specific?

The hourly load curves of the selected household in each season are shown in Fig. 28. Figure 28 shows the demand for electricity in four months on an hourly basis. Can you explain this diagram to...

08 February 2021 657 16 View

Why is there cloudy white solution in flavonoid test tube?

I do flavonoid test for biscuit incorporated with muntingia fruit powder. Sample extracted with metanol (1:10 ,w/v), macerated 24h, sentrifuged and filtered. Supernatan used for analysis. I have...

08 February 2021 6,779 4 View

How define one baker unit(BU) is the amount of hydrogen peroxide catalase ?

07 February 2021 2,006 1 View

Which one is better for storing energy: Fuels synthesized from CO2 or Hydrogen?

Based on educated guesses, which one of the following promises a better future for storing energy for grid scale, automobiles etc as opposed to batteries: 1) Fuels synthesized form CO2 2)...

02 February 2021 8,741 6 View