Hi Amin,

I am not sure of the technique or assay you are wanting to use for assessing Clotrimazole. What concentration of stock are you preparing? What assay are you going to be using? Biological matrices or prepared in solvents. A standard stock solution that I would normally prepare for this would be at 1 mg/mL.

I would suggest using 100% ethanol as a solvent for this, Clotrimazole should be soluble in ethanol at (10 mg/mL). it is possible to use ethanol/DMSO (50/50 v/v) if this helps your solubility going forward, the solution would have a reduced capacity for Clotrimazole but will easily incorporate a 1 mg/mL concentration.

These should be compatible with biological analysis. you would have to double check if it works for your application.

I hope this helps.

Drug solubility sometimes depends on pH. try to change pH it may affect solubility. you can use Chemicalize site ( chemicalize .com) to know what happens to drug solubility with different pH. Also, you can use hot water instead of cold water.

Hi Mark,

Thank you for your answer and the above-mentioned information about clotrimazole's solubility. I would like to use clotrimazole as a daily oral treatment for mice. I've seen that it is soluble in both Ethanol and DMSO, with higher solubility in ethanol. I was not sure if ethanol can be administered orally to mice. Accordingly, I tried dissolving it in DMSO, but it didn't work. However, many have done it before. I also tried heating it, but precipitation was still there. Do you have any recommendation for such an application?

Thank you again.

Hi Michael,

I haven't checked the PH, but thank you for your recommendation. I'll take this into consideration. The drug itself isn't soluble in water, but I've tried to heat the drug after adding the solvent (i.e. 1% DMSO).