Does anyone have information on cornea epithelium segmentation?

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Wolfgang H. Muss

Dear Shumoos Taha Hammadi,

If you want to get any serious reply or help with your matter I suggest you attach at least a .jpg or .bmp out of your recent analysis/analyses to this thread.

It makes no sense to discuss anything about not knowing what your problem really is (specimen: ok: we know about EYE (animal-human?) / Cornea epithelium.... but nothing about methods to get sections(?): paraffin-, cryo-, semithin plastic /resin sections, stain(s) used, method of image acquisition etc, etc.).

Sorry to bother you with my request, but hopefully you'll answer some of my questions.

Shumoos Al-Fahdawi

Thanks Dr. Muzhir Al-Ani for your answer, actually I tried to work on threshold but I didn't get good result.

Thanks Dr.Wolfgang H. Muss. I'm working on EYE human and I should detect the nerve and cells, but at this stage just on nerve segmentation, and I'II attach to you some picture after enhance it.

I hope you can help me.

Luminita Moraru

Do you use certain pre-processing operations? Your images are very intensity inhomogeneous, so you should use some operations in order to improve they, before the segmentation.

thanks for attaching some images. Nevertheless I am guessing still:

i) fluorescence or histochemical stain / FLUOR / Normal-transmitted light (e.g.darkfield, reflectance) microscopy?

ii) staining method

iii) specimens: paraffin, cryo, resin sections, whole specs native (these all would be possibilities).

iv) I would second (indeed not knowing about the origin of images, hardware, software for image processing, if any) Luminita Moraru's opinion as above....

I am sorry about not being able to help again.

Swapnil P Bhatia

I think Fiji/ImageJ should work well for this. There's also the Cell Profiler from the Broad Inst. Most of them have pre processing and cell counting pipelines or modules built in.