How could the concentration from RNA extraction is really low?

Hello everyone, currently, I'm trying to do RNA extraction from seeds of Arabidopsis. I'm using the protocol below: RNA isolation from siliques, dry seeds, and other tissues of... I use about...

17 February 2021 7,583 4 View

How do I measure Arabidopsis germination rate?

Hi there, I'm looking for a way to measure the Arabidopsis germination rate, like what media I should use to plant the seeds and what indicator I should see for germination. I'm planning to...

02 February 2021 4,785 3 View

SDS-Gel stacking gel polymerization issues?

Can anyone provide any insight as to why my Tris/Gly SDS-Gels are polymerizing slowly? This especially applies to my stacking gel (image attached) both 4% and 6%. The stacking gel wells polymerize...

24 January 2021 3,791 4 View

How to use back-trajectory analysis (HYSPLIT) to assess air parcels at breathing/monitor height?

Hi there, I'm currently monitoring air pollution in Grenada, West Indies and as part of my project I have identified four scenarios in which I would like to use a back-trajectory analysis...

27 December 2020 730 4 View

Origin of the term "transposition" used in photovoltaic performance modeling?

In PV modeling, the process of elevating GHI data to a tilted surface is often referred to as "transposition". However, strictly speaking, "transposition" in mathematics refers to rearranging a...

22 September 2020 767 3 View

Help figuring out why only lifted cells express eGFP after transduction?

I'm attempting to transduce HEK293T cells with a lentivirus expressing eGFP. I have been observing a low transduction efficiency and after a 24 h transduction the only cells expressing eGFP are...

03 September 2020 9,491 5 View

How to stimulate PBMC-derived monocytes with TLR7 and TLR8 agonists?

I'm trying to stimulate human monocytes from fresh PBMC isolations with 2ug/mL imiquimod and 2ug/mL ssRNA40. I do this in 10% FCS complete RPMI for 18-20 hours before staining and analysing CD14+...

10 August 2020 7,095 1 View

What are the independent, intensive parameters for multicomponent mixtures (and how do I determine them)?

Hi all, For different problems in gasdynamics/thermofluids, different thermodynamic inputs are most convenient for expressing and solving the problem. For example, if I have a stagnation process,...

23 June 2020 1,085 2 View

What literature should I read to start understanding flow batteries?

I need recomendations on the most core and fundamental materials/literature to start understanting flow batteries from scratch so I can migrate to membraneless flow batteries. Hence, I will also...

09 June 2020 6,270 3 View

Any recommendations for a high-quality, contract basis, academic illustrator for a paper in Nature Climate Change?

We looking for a high-quality, contract basis, academic illustrator for a paper in Nature Climate Change, any recommendations? Proven track record important.

26 April 2020 7,427 8 View

Helicoverpa armigera eggs are not hatching in lab condition, why?

Dear RG friends, I am providing a complete H. armigera diet for larvae and maintaining the eggs in the growth chamber as well as in-room conditions. But since three months, eggs laid from the...

21 January 2021 5,546 32 View

Triggering feeding of beetles / how to make them hungry?

Hey, I am working with Psylliodes chrysocephala feeding on oilseed rape. In feeding trials I often have the problem that a huge portion of the beetles do not feed at all. In clip cages I observed...

19 January 2021 9,851 6 View

Detection of insect eggs in stored and processed products?

Currently, in order to identify insect eggs in stored or processed products (such as contaminated flour or wafer samples), staining was performed based on the method mentioned in the below...

15 January 2021 6,362 5 View

Has anybody a protocol to determine the AChE activity in larvae zebrafish brain?

Even following the instruction of Karnovsky and Roots (1964), I still do not know how long I should fix the larvae and in what fixative. Could anyone help me? Thank you!

14 January 2021 385 2 View

Can any one suggest the name of Litchi insect/predator?

Is it a type of Ambush bug or what? Thanks

14 January 2021 9,381 3 View

Which is the most recent and updated classification of Class: Insecta?

Based on the many publications from several decades, we came across many classifications of Insects. Among them which one is more updated and approved classification that can be used for research,...

05 January 2021 3,668 9 View

How to inject RNAi into moth larvae?

Hi, I would like a detailed explanation/photos/video How to do it in moth? (I work with Helicoverpa armigera and Spodoptera frugipreda larvae)

04 January 2021 8,194 4 View

How can we say a species of insects got extinct?

02 January 2021 4,232 10 View

Help with insect identification?

Could someone help me identify the insect in this picture?

31 December 2020 2,109 5 View

Could you suggest me some suitable pesticides for insect larvae in spirulina farm?

Hi everybody , I hope all is well. I've got a question Could you suggest me some suitable pesticides for insect larvae in spirulina farm? Thanks

30 December 2020 2,297 7 View