Does the Illumina Eco Real-time PCR system require calibration for HRM (SNP detection)? The system manual mentions 'no temperature shift or calibration required'. However, I need to be certain before designing my experiments.
Any help would be appreciated.
Aishwarya Amirdharaj
Hi,
Yes the system is already calibrated thus you are only required to set your experiment such as temperature,duration etc which you should be careful and set accordingly to avoid exceeding beyond their system limitations also.
Taking care on DNA samples quality,MgCl2 concentration, Dye concentration etc would help get better results.
All the best...
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