I am investigating the stress of capture and confinement on wild animals. Plasma aptoglobin levels are an indicator of chronic stress and handling procedures in cattle and pigs. This new ELISA is supposed to be deer specific, but is rather expensive so I need affirmation that it really works.
Dear Neville,
I have no experience with that ELISA. But you might know that haptoglobin binds hemoglobin and, thereby, induce / stabilize a peroxidase-like function of hemoglobin that you can measure independently from species. This function is used by commercial kits like the kit from BioRepair:
http://www.biorepair.com/diagnostics-human-veterinary-medicine/vet--diagnostics-:c8.htm
This kit uses a 96-well plate for measurement. But if you want to save money (and have good eyes and no tremor) you can adapt the kit to a 384-well plate. The only prerequisite is that you have no hemolysis in your samples!
Best regards,
Frank
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