Does anyone have experience with the production of iPSC by the episomal method?

More Sandro Orru's questions See All

Research about Gamification by board games?

obrigado pelo comentário.

21 September 2023 6,353 5 View

Is this a good mixed-methods research aim?

I have conducted a multiphase mixed-methods research, through which i developed, implemented and evaluated an injury surveillance system that was tailored for stakeholders as a practical injury...

13 August 2023 2,641 2 View

What is the shelf life of organic solvents used for LC-MS? Is there any valid data to substantiate device manufacturers recommendations?

Does anyone know any official document or scientific publication logically explaining why I should replace for example HPLC-grade acetonitrile or methanol (pure, or with

14 April 2023 5,796 4 View

Qualitative strands ‐ Can they be connected?

Mixed methods approach: the initial qualitative findings were used to build an intervention, from which I collected quantitative results (seq exploratory). The last qualitative strand was used to...

28 August 2022 2,538 1 View

Anova, Manova, Ancova, Mancova, or Correlation, or something else?

Hello, I would like to ask you for your opinion. Recently, I conducted a research study to find out whether or not there is difference in moral reasoning between people with BA, MA, and PhD...

09 February 2022 6,608 5 View

How can I model the contact between a truss and a solid in Abaqus ?

I'm actually trying to connect the 2 anchorage points in an UNBONDED, prestressed concrete beam. I'm a newbie in Abaqus, please help me describing the little steps. Thank you in advance!

21 October 2021 3,100 3 View

Expansion of a function of two variables x and y in terms of x/y ?

Dear Colleagues, I would like to expand the function (1-x)/(1-y) about (x=0; y=0) with x/y argument of the expansion. x and y are definite positive. Any suggestion? Thanks Sandro

20 September 2021 6,423 4 View

Longitudinal mixed methods design?

I'm currently collecting longitudinal repetitive quantitative measures (survey tool has been developed based on initial qualitative data - exploratory sequential) from the same population (n=39)...

02 September 2021 9,785 5 View

Is this an explanatory sequential design?

I am making use of a multiphase mixed-methods study to develop, implement and evaluate an injury surveillance system. In the first study, I am making use of an exploratory sequential design,...

25 January 2021 3,092 1 View

How to approach a system of three autonomous ODE in the phase space?

Dear colleagues, the problem I am analyzing is a gravity current in viscous-buoyancy balance propagating towards the corner of a fracture that is reducing its width according to b(x)=b_1 x^k,...

31 October 2020 511 0 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

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04 August 2024 1,200 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Jun Wei

I reprogrammed human fibroblast cells to iPSCs many times, and using both lipofectamine and Lifetech Neon electroporation system. Yes, you can get colonies from both methods. Life tech claimed 3K is more efficient, but I have to say, based on my experience, lipofectamine 3K is less efficient than electroporation. There is another issue, different cells have different preference, you should try.

Sandro Orru

Thank you Jun for your prompt reply very useful