I work with primary human T cells and have always used Amaxa. The problem is that amaxa is not so great with big constructs.
Sorry, but we have never used that system. I'll check in my department if someone has experience with it and I'll get back to you.
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
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