28 August 2020 2 7K Report

I am considering of using the original one from Brooke-Gunn and Ruble (1980), but maybe there is something better? I am trying to analyze the attitude of athletes towards menstruation and its further impact on their self-efficacy and performance.

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What is the cause of this aggregation just bellow 70 kDa mark?

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21 July 2024 5,128 4 View

How many search hits is enough for scoping review?

I am deciding on search terms for a scoping review. I am planning searching 3 multidisciplinary databases and currently getting about 5000 hits from my search terms in the first database. What do...

19 June 2024 7,417 0 View

RQ results discrepancy?

Hello, I'm relatively new to qPCR and have been utilizing a manufacturer's analysis software for my experiments, including one-way ANOVA for statistical analysis. However, I've encountered a...

14 May 2024 3,307 3 View

What test should be used for comparing multiple groups of categorical variables with counts per group <5?

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13 May 2024 1,425 2 View

Problem: cell aggregation with Cytofix/Cytoperm BD Biosciences?

The challenge encountered lies in cellular aggregation and clumping subsequent to incubation with Cytofix/Cytoperm BD Biosciences. Despite attempts to mitigate the issue through pipetting, the...

12 March 2024 3,926 0 View

Can anyone help me identify a cell culture contamination?

cell culture: HEK293F medium: BalanCD, without antibiotics

30 March 2023 8,850 1 View

How can I prepare Rietveld Refinement for two compunds?

In my case: LiBH4 - LiBr in different molar ratio. I have the observed patterns (measured by XRD technique) for this mixture of compounds but I don't have .cif file or any reference to compare...

03 March 2023 2,640 4 View

How to eliminate dark background in DAB stains of mouse brain cuts?

Hi, I recently started doing a lot of IHC and I’ve been staining mouse coronal section (not in parafin) with 3D6 antibody to visualize plaques. My problem is that the background is too dark and...

02 December 2022 6,768 1 View

What is the best protocol to stain kisspeptin in the mouse brain?

Dear all, Have you ever used kisspeptin antibody antiserum AC566 rabbit anti mouse? I wanto to try immunfluorescence on the mouse brain tissue but I don't have much expereince with this method....

21 October 2020 5,218 0 View

Can mycoplasma contamination in primary neuronal culture interfere with neuronal growth?

I am doing primary neuronal cultures (mix of neurons and astrocytes) from mice. One year ago we moved and changed labs and since that time I am not able to grow nice neurons (either they don't...

30 September 2020 2,731 1 View

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Hello In your opinion, which is better: Study Microprocessing first, then Computer Architecture, or vice versa, and why?

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