Share a copy of RaFD Dataset?

I tried to get this dataset from the original link but no one approved my requests for weeks. Can someone share with me a private link to use? You can send it to my institutional email:...

14 February 2021 1,908 1 View

Help with Amyloid beta detection protocol (western-blotting)?

Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...

11 February 2021 2,513 3 View

Does viral RNA extraction from nasopharyngeal swabs require pre-centrifugation with supernatant collection?

06 February 2021 323 3 View

Aspen plus (hydrometallurgy set-up) & reactions solid-liquid ?

I have to simulate a hydrometallurgical process where there are the following phases: - leaching (solid-liquid reactions) - cementation (solid-liquid reactions) - electrowinning (energy ) -...

30 January 2021 9,225 3 View

How to prediction non-visible parts of microscopic pictures?

Dear all, I like to ask if someone has experience with evaluating pictures towards the prediction of non-visible parts. I have confocal-laser-scanning microscopic pictures of yeast cells and want...

28 December 2020 4,309 11 View

Coronal or Sagittal mouse brain sections to visualise vessels?

Hello, I am a first year PhD student and I am working on Cerebral amyloid angiopathy (CAA). I would like to investigate the presence of my proteins of interest in a CAA/Alzheimer's disease mouse...

05 December 2020 907 3 View

What is the cleanest / best way to handle different software dependencies / configurations?

Dear RG users, I noticed that several toolboxes developed for python are compatible with some specific releases and not with others and in general there is a precise set of tools defining the...

30 November 2020 4,545 5 View

Why maximum allowable limits for toxic metals or heavy metals in agricultural soils vary among countries and institutions?

For example, the maximum allowable limit for USEPA in agricultural soils are very low when compared to Tanzania's maximum allowable limits see figure below. Does this can affect the management...

23 November 2020 5,052 10 View

What is the best way to get organoids of the same size?

I would like to get organoids of the same size. We filter organoids using a 100 um filter and then pass that filtrate through a 40 um filter followed by flipping the 40 um filter. Then we wash the...

22 November 2020 9,391 3 View

How to match Matplotlib color mapping in a R ggscatter plot (or ggplot)?

I created this shinyapp and am having a hard time seeing the image I thought I was...

13 October 2020 223 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

What are the ranges of four diffterent poles of butterworth filter for ECG signal?

Hi, Can anyone please tell me what are the ranges of four different pole orders such as first order, second order, third order and fourth order respectively of butterworth filter for ECG...

23 February 2021 2,554 3 View

Are we using now IT technology effectively?

I discover now two my papers, which are more then 30 years old and which are describing something, what was done in years 1985 -...

21 February 2021 5,008 3 View

How to use Remote sensing and GIS applications for mapping the available medicinal plants within the locality/municipality?

Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis...

21 February 2021 374 5 View

Can I use the CHARMM GUI input files for Gromacs in HPC?

Good day everyone! I am a Biochemistry undergraduate in the Philippines and to be very honest, we have no background in bioinformatics since we really focused on wet lab techniques + theory. Due...

15 February 2021 3,422 4 View

What are the popular techniques for pre-processing measurement data in Smart Grid/Power System?

The data collected from various measurement devices often contains erroneous values - such as noise, missing values, etc. What are the popular techniques for pre-processing measurement data in...

14 February 2021 4,642 2 View

Is there a way in which microglia can be extracted from mice without killing them ?

I'm aiming to use an adoptive cell transfer approach, with microglia in mice with ALS. So far all the protocols I have seen, require sacrificing the mouse. I'd like to re-infuse the microglia back...

11 February 2021 9,509 1 View