Dear this is my special snd experience work how can I help you with my pleasure

Dear sir,

I am thankful to you first of all. what are the stereogical parameters can take from the testis?

what is the procedure have to follow for the stereological study on testis?

please do needful

Mahesh Babu,

The integrity and structural modifications occuring in intratubular sections could be evaluated as originally described in details in our laboratory by Milazzo et al. (2008) : https://academic.oup.com/humrep/article/23/1/17/563606

Then, this Score was a little modified (Dumont et al., 2015) : Article Assessment of the optimal vitrification protocol for pre-pub...

Briefly, a score between 0 and 5 was given for (i) nuclear and (ii) epithelial alterations, with 0 representing the complete absence of alteration and 5 representing the most severe alterations. Therefore, the global score for each seminiferous section varied between 0 and 10 for the fresh controls and frozen‐thawed testicular samples.

The structure of a seminiferous tubule in sections was judged to be intact when epithelial and nuclei scores were both without any morphological alteration.

For each testis, the global score of peripheral, central and global areas could be determined independently by taking the mean of the scores of 30 seminiferous tubules. "Morphological alterations" correspond to the global scores normalized to 100 (originally measured on a scale of 10).

Hoping that this complement reply will be useful,

Dear community of RG, feel free to add modifications & precisions to my Answer!

Ludovic

I work on quantitation of spermatogenic cell yeild and leydig cell indecies, correction of Sertoli cells and abrocrobies formus , quantity of voleum dencities of of seminiferous tubules an other paramerters determine what type of these I can help you with my pleasure

Dear Mohanad,

Thank you so much for your response, i want to study how to quantify the spermatogenic cell, leydig cell and sertoli cells.

thank you @

Hey Mahesh, You can quantify various cell types by performing a blinded scoring analysis, by point counting method for instance. You can stain the testicular sections with germ cell, leydig cell and Sertoli cell specific markers. In addition, you can perform morphometric analysis of the testicular sections, score the sections based on the morphological variations (for example, epithelial arrangement of the tubules, tubular appearance, immune cell infiltration within the tubules, presence of the most advanced germ cell types).

Thank you swati sharma,

can you suggest any reference article

Well I would mention some recent ones from my group, which may give you some idea for different analytical approaches which could possibly be employed: https://www.frontiersin.org/articles/10.3389/fendo.2018.00467/full

https://academic.oup.com/molehr/article/23/3/141/2960711

https://academic.oup.com/endo/article/148/11/5541/2501661

Thank you so much madam

I work in failed reproductive system and detriment fertility index what type of these can help

What is important setrogical parameter in artical can use in femal ?

Not using female