I want to purify my secreted his tagged protein from condition medium of HEK293T cells using HIS SELECT AFFINITY GEL OF SIGMA - BATCH method (NOT column chromatography). Does anyone use this method? I want a good protocol for it.
Nafiseh - Try GE's Ni Excel resin. I have had good success with the use of this resin over several others. GE sells them in 1mL and 5mL HiTrap formats.