Does anyone have experience with PCR of the quinolone resistance determining region (QRDR) of S. aureus?

21 November 2013 0 2K Report

Specifically grlA, grlB, gyrA, and gyrB genes. Could anyone clarify the protocol that you use for this?

Badges
Science topic

Similar topics
Education
Students

More Zachary Tyler Tackett's questions See All

Does this corrosion exam question read well and do you see the solution pathyway?

Hello, I am teaching for my first time this semester. In writing my exam, I felt pretty good about most of my questions except one. I was wondering if anyone could provide feedback on their...

28 February 2024 9,051 1 View

What should I do with missing data when averaging variables?

I am performing a cross-country regression analysis with a sample of 101 countries. Most of my variables are averages of annual data across a period of 7 years. Every one of my primary variables...

20 April 2023 4,337 3 View

What terms are the International Monetary Fund's International Financial Statistics in?

I am using certain IFS variables in my research, particularly "Supplementary Items, Direct Investment, Net Incurrence of Liabilities (Excluding Exceptional Financing), US Dollars" and...

30 March 2023 5,534 4 View

How do we decide on the sample size in a particular study?

I plan to study teachers' perception on M-Learning using a survey. How many teachers should be involved in the survey?

06 February 2023 8,285 8 View

What causes this IHC artifact?

Has anyone seen this immunohistochemistry artifact, and do you know what causes it? The artifact appears as patches of chromogen throughout the tissue sections. I have seen this artifact in...

11 December 2022 744 2 View

I'm having a lot of trouble dissolving a phthalate/vehicle combination in cell culture medium for in vitro toxicity assays; Can anyone provide tips?

I work with a very hydrophobic phthalate (DEHP). Visually, it appears to be miscible in DMSO and 100% ethanol but following addition of the solvent/solute combo to culture medium (RPMI-1640 +...

08 September 2022 9,900 0 View

ANSYS Fluent - How to get zero species diffusive flux through reaction wall, or specify species flux through a wall?

I am using ANSYS Fluent to model a 2D microfluidic channel. The channel is 50um tall and 1.5cm long. This model is of cells on the bottom surface consuming oxygen and producing dissolved waste...

08 August 2022 627 0 View

What to do with 100% datapoints when conducting a Logit transformation?

I have a panel dataset of 120 different countries measuring a variable over three periods. This variable indicates the percentage of 1000 respondents in each country that answered yes to the...

01 February 2022 1,284 7 View

How do I prepare zebrafish embryos for homogenization?

Hello, I am wanting to do an RNA isolation on zebrafish embryos using the PicoPure RNA isolation kit. I was wondering if anyone could describe the homogenizing procedure for zebrafish embryos...

16 January 2022 6,773 0 View

How does subsequent heating affect properties of previously cured epoxy?

If epoxy is cured at low temperature to 50% degree cure, then further cured to 100% at higher temperature, how will that be different than curing to 100% at the higher temperature? Similarly, how...

20 July 2021 8,007 5 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

What is the best way to drop a plasmid?

Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...

25 July 2024 2,532 3 View

Someone have the key or the installer of the program Mx pro 3000 (PCR real time)?

I need to install this program

25 July 2024 4,756 0 View