Does anyone have experience with, or literature on, bone plow or rake phenomenon using locking plate fixation ?

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Property of the harmonic mean?

The harmonic mean $$ of two positive numbers $x_1$ and $x_2$ can be written as a weighted average $=w_1 x_1+w_2 x_2$ where the weights are $w_1=x_2/(x_1+x_2)$ and $w_2=x_1/(x_1+x_2)$. As such...

17 April 2024 2,612 11 View

Papers presenting analyses and decisions with NO data: what do you think?

Many times I read interesting papers presenting analyses and decisions on Real Cases (with data) to support the “theory” BUT NO data are provided. In such cases there is NO possibility to analyse...

12 September 2023 8,708 1 View

Average Run Length? How to compute it?

On Wikipedia I found about ARL: Even when a process is in control (that is, no special causes are present in the system), there is approximately a 0.27% probability of a point exceeding 3-sigma...

06 November 2022 6,777 8 View

Does someone suggest where I can find the appropriate tools for performing a Quantum Transmission Photon Test similar to BB84?

I am looking to purchase the set of equipment related to the QKD BB84 performed in the laboratory. So, I will need a set of: - Polarization Filters - Photon Detector - Laser (one-by-one photons...

06 April 2022 5,152 0 View

Datasets on Semantic Similarity between Words/Concepts?

Are there available datasets quantifying semantic similarity for word pairs in English? Many thanks for your help!

08 January 2022 2,095 11 View

Can anyone help me with COMSOL RF boundary mode analysis?

My final goal is to propagare an RF surface wave on a purely reactive surface (Zsurf). First of all I run a mode analysis on a 2D rectangular geometry with a surface current (emw.E/Zsurf) on the...

16 November 2021 7,013 0 View

Single or multiple Voltage thresholds for successive APs in current clamp?

Hello everybody. We are trying to write a script to analyze current-clamp electrophysiological traces. I write asking for help because we stumbled on an issue that we couldn't really...

09 April 2020 8,958 0 View

Why my mESC won't attach to the plate?

I am culturing mESCs on 2i media. The cells are actively proliferating, but they barely attach to 0.2% gelatin- coated plates. Does anybody ever experienced similar issue and have some suggestions...

15 January 2020 5,936 6 View

Could anyone confirm please my identification of this species of freshwater bryozoans?

Hi, I attach an underwater picture of a colony of freshwater bryozoans. I identified it as Cristatella mucedo. I took the picture in a small natural lake in Northern Italy, in the Province of...

03 December 2019 7,882 2 View

Etiology of violence?

Have you collected data on the possible etiology of violence in offenders? Are there any recurrent biographical data in your sample? Let me know if you are interested in collaborating or at least...

01 August 2019 9,840 0 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Can you recommend a 96-well plate for minimal cell adherence to the wells during phagocytosis experiments?

I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...

04 August 2024 5,228 4 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

Found this object in the cell culture - do you have any suggestions what it might be?

Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...

01 August 2024 2,250 2 View

Publishing a checklist?

Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...

01 August 2024 6,134 2 View

Culture fibroblasts with or without FGF2?

I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...

29 July 2024 3,030 2 View

Are you interested in contributing a book chapter on biomaterials in Orthopaedics?

I am editing a book for Springer Nature on recent advancements and future trends in biomaterials for orthopedic and trauma applications, with a publication date slated for early next year. If you...

29 July 2024 5,015 9 View

HEC 1A & HEC1B Cell Lines?

Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...

20 July 2024 4,143 2 View