Is it suitable to stabilise human blood cells by RNA-Later to perform DNA expression studies?

Human blood cells stabilised by RNA-Later to perform DNA expression studies. It is a good way to store white blood cells to perform subsequently the isolation of RNA? Or is it preferable to store...

23 June 2015 3,340 3 View

Can someone give me a suggestion on the cloning procedures?

11 March 2015 1,728 4 View

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I have a list of genes annotated with InterProscan. I would like to summarize the results plotting the GO category and look at which major function or biological processes those genes are involved...

12 August 2014 455 2 View

Who knows sites hosting free samples of images with known clusters inside to test clustering machine learning models?

I have several clustering models to be tested and validated on images for which clusters inside are well known.

23 March 2014 7,277 2 View

Could somebody please give me the nucleotide sequence of the TEV protease recognition site?

I would like to use this sequence for TAP of protein complexes.

17 February 2014 7,498 7 View

Which activities should be the most important for academic people?

Normally an academic dedicates do some hours per week to research, some to teach and some to administration of the Department or Faculty. Some hours are also dedicated to external work, as...

01 November 2013 4,783 3 View

Can anyone advise on antibodies against "histone locus bodies" that work in imunofluorescence?

I am trying to identify histone locus bodies in a prostate cancer cell line (PC-3)

09 July 2013 280 3 View

Hek-293 stable cell line problems?

I am having a problem with the selection of my HEK-293 cells. I started the selection with G418 two months ago and I still have in my flask untransfected cells that don't die. What can I do? Try...

20 June 2013 1,849 19 View

What are the best markers for the identification of Neutrophils, eosinophils and circulating dendritic cells in whole blood by flow cytometry?

I need to study these cells in whole blood samples. I normally use the protocol "Lyse-no-wash". It could be used to study these cells, too?

05 April 2013 5,771 10 View

Are the telomere length different in human skeletal muscle upper limbs respect lower limbs?

Is there any evidence that telomere length in human skeletal muscles is limb specific?

03 April 2013 8,558 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View