What colour is a resorufin solution at pH < 6.1?

The redox dye resazurin (dark blue) can be reduced in aqueous solution at pH > 6.1 via resorufin (pink) to dihydroresorufin (colourless). What colour is the resorufin solution at pH < 6.1?

21 January 2021 8,442 2 View

We use Thermo Scientific Superfrost plus gold coated slides. Why our cryo sections of dry fruits do not stick to the slides?

We use Thermo Scientific Superfrost plus gold coated slides. Why our cryo sections of dry fruits do not stick to the slides?

13 January 2020 1,215 1 View

My mysterious red dye may not be neutralized before HPLC/MS as it is only stable between pH 12.5-12.9. Who could help with equipment tolerating pH13?

More information in https://www.chemistryviews.org/details/education/10821368/150_Years_Alfred_Wohlk.html

04 July 2019 1,409 1 View

Are smaller insect species more sensitive to residual insectides?

Alarming declines of insects elicited a vivid public debate, particularly in Germany (#Insektensterben), but also in many other countries (see...

06 September 2017 7,975 4 View

Someone knows a company that offers gene synthesis in expression plasmids for use in plants?

16 May 2016 8,212 3 View

Which flower signals are honest?

There are some reports about honest floral signals, e.g. flower colour change in many flowering plants correleted to nectar production, size of Dalechampia bracts indicating the size of a resin...

25 April 2016 1,418 5 View

Which pan trap colors are attractive for bees?

Dear All, I want to learn about pan trapping of bees and the most attractive colours. Does anybody know published spectral reflectance curves of pan trap colors? Can anybody explain the term...

08 March 2016 7,710 44 View

How does flower suntracking correspond to bee foraging behaviour?

Many flowers show heliotropism and the flowers are sun tracking. The discussed benefits of sun tracking flowers are heating up the flowers for better pollen germination and seed production, and...

26 January 2016 9,316 5 View

Does it make sense to compare linear regression slopes in the Murmansk region (Russia) using air temperatures from gridded E-OBS data?

12 June 2015 8,483 14 View

Why do some researchers neglect multiplicity in the calculation of statistical significance?

14 February 2014 786 3 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View

Is it possible to use maize mature seed to do transformation for maize transgenic study?

Almost all research reports which i ever seen work on Early mature embryo for Agrobacterium mediated maize transformation. In other grass families like rice, we can use mature seed and what do...

17 February 2021 1,434 13 View

Dealing with BMDM seeding and RNA extraction ?

biomedical master student here , i am dealing with mice BMDM and would like some help , i seed 0.5 x 10^6 cells/per well in a 6 well plate but when i extract the RNA i get a very low yeild , i...

15 February 2021 1,102 5 View

Is secondary metabolite production more at vegetative growth stage or during reproductive stage?

Hi, I am working on the effect of heavy metals on a medicinal plant by pot experimentation. Analyzing the effect of heavy metals on secondary metabolites is one of the parameters. I will be...

11 February 2021 6,683 6 View

Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View

Seeds harvest from white clover by selfing?

I tried to get seeds from white clover by self-fertilization but I could not get the seeds. As you know the white clover is generally fertilized by crossing. I wanna get the seeds by...

04 February 2021 9,152 4 View

What is the best method of preparing cell supernatant samples for a Luminex assay to detect cytokines ?

I am planning to run a Luminex assay using cell supernatant to assess cytokine secretion by tumor cells. I came across different methods in literature; some suggest seeding in complete media and...

03 February 2021 4,867 3 View

How do I measure Arabidopsis germination rate?

Hi there, I'm looking for a way to measure the Arabidopsis germination rate, like what media I should use to plant the seeds and what indicator I should see for germination. I'm planning to...

02 February 2021 4,785 3 View