Is there any method available for isolation of mouse primary hepatocytes without routine initial perfusion steps? I was wondering if is it possible to isolate hepatocytes when liver is outside mouse body. Once I perfuse mouse then it won't be possible to use the same animal liver for other purposes than just to isolate primary hepatocytes if I do it traditional way. I am working on transgenic mouse where I have limitation of number of animals. Can anybody provide some valuable opinion?