Does anyone have experience with genetic transformation of the bacterial genus Aneurinibacillus?

02 March 2023 1 907 Report

I am trying to genetically transform a species of Aneurinibacillus. I have tried protoplast transformation, electroporation, and natural transformation, but have not been successful. Does anyone have a good protocol for this? Thank you.

Robert Adolf Brinzer

You trying to transfect a plasmid known to work in this species? Have you checked the plasmid has a compatible origin and is lacking restriction sites for which your species has restriction enzymes?

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