Hi, I would like to know if anyone has tried DNA recombination in P. aerophilum. I would like to KO a gene but I don't know if tools are available for this organism. Many thanks.
yaa it is possible as it use both organic and inorganic substrate for respitration , making it to stress conditions on growing on sucrose and try pKO3 vector
which may help u they are other non cromosomal vector which need sucrose substrate ans remember to chose the expression vector.