I need to have information about the % of release to be considered to assess that there is cytotoxicity. I work with MSC as target cells.
I am trying to refine my data in full prof and my chi2 is 0,834E11 and everytime i try to refine the data the plot deviates further from the actual data and the chi2 doesnt change. The first image...
02 June 2024 5,805 5 View
Which is the best method to isolate Trichoderma in lab? rice traps or PDA or a different method?
19 March 2024 8,154 1 View
We are currently trying to transform Bacillus licheniformis (environmental isolate) with plasmid pWH1520. Unfortunately, we barely get colonies growing on our plate regardless of the...
18 August 2023 411 4 View
I would like buy enzymes with not an high purity but in an huge amount, 1g or more.?
06 January 2019 7,527 3 View
Im working on fungus that atack to species of wild plants who's are very important in the paramo ecosystem, so I want to know if insects can be mechanic vectors of that fungus. The problem is...
29 May 2018 3,732 2 View
I have tried all protocols and I can´t avoid degradation so I don't get any amplicon in the PCR reaction. I tried trizol, regular extraction from wormbook, different lysis buffer, etc. Does...
03 October 2017 1,880 3 View
I will be researching an structural change (from hierarchical to holocratic) in a company with the ethnographic observation method. I have no experience with this approach and would be glad to...
19 March 2017 3,492 7 View
Hi everybody! I try to write a little article about the new trend of Reinventing organizations and I am searching for pros and contras of this new methods. I have found it really hard to get any...
20 June 2016 4,622 4 View
Hi there, I have some contradictory results on validation of miRNA microarray results by qPCR: LNA primers from Exiqon have not shown expression of the selected miRNA (Ct similar between samples...
31 January 2016 6,531 1 View
I´m working with peanut plants and heavy metals (Cd and As) as environmental stress. I cannot find any research about Arsenic and peanut plants. Does anyone know the oxidative effect of this...
13 September 2012 2,921 0 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.
17 July 2024 3,532 0 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?
15 July 2024 1,598 3 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...
10 July 2024 6,830 1 View
Hi, I would appreciate professional and technical insights regarding primary cell culturing. I've been working on optimizing my primary cell culture, and while it is successful at times, there are...
10 July 2024 4,918 2 View