Hi Norulsazyani, I have been using CMC 1% for evaluating  Cx cellulase. if you prepare it in a dish then you can add hexadecyltrimethylammonium bromide 1% and you will see the area where the enzyme has metabolized the CMC. I did not get if you want to produce or you want to detect the enzyme...

Dear Norulsazyani,

In addition to what suggested by Sabrina, the following publications describe screening fungi isolated from historic Discovery Hut on Ross Island, Antarctica for cellulose degradation & Isolation and Characterization of Rapid Cellulose Degrading Fungal Pathogens from Compost of Agro Wastes that might help you in taking a decision how to proceed with your project.

Isolation of fungi

Fungi from the samples taken in January 1999 and December 1999 were isolated on the following media: YM agar (yeast extract 0.2%, malt extract 1.5%, agar 1.8%),

Medium 4 (yeast extract 0.2%, malt extract 1.5%, agar 1.8%, chloramphenicol 0.2 g l -1, streptomycin sulphate 0.1 g l -1 ) for isolation of streptomycin resistant fungi

(Harrington 1981), Medium 6 (yeast extract 0.2%, malt extract 1.5%, agar 1.8%, chloramphenicol 0.2 g l -1, streptomycin sulphate 0.1 g l -1, cycloheximide 0.4 g l

-1 ) for isolation of cycloheximide resistant fungi (Harrington 1981), Medium 7 (yeast extract 0.2%, malt extract 1.5%, agar 1.8%, chloramphenicol 0.2 g l -1, benlate 0.06 g l -1, streptomycin sulphate 0.1 g l -1, lactic acid 2 ml) for selection of Basidiomycetes, and Vogel Bonner minimal medium (-25% glucose, 2.0% agar,

20 ml VB concentrate/litre (50% K2HPO4 (anhydrous), 17.5% NaNH4PO4 . 4H2O, 10% citric acid . H2O, 1% MgSO4 . 7H2O in 670 mls distilled water) for the selection

of slow growing fungi (Vogel & Bonner 1956). For fungal isolations wood samples were surface sterilized by soaking for one minute in a 5% hypochlorite solution,

followed by two rinses in sterile, distilled water, sliced and cultured on a variety of enriched and semi-selective media prepared as agar plates. Organic material samples were cut with a sterile scalpel and placed onto culture media; swab

samples were wiped over the surface of the media; wood scraping samples were aseptically placed onto the media. The agar plates were then incubated at 48C, 158C or 258C for up to six weeks. Organisms growing on the agar plates

were transferred by subculturing from hyphal tips, colonies or spores to fresh YM agar plates. Fungi from the samples taken in December 2000 were isolated by the following method: a small amount of each sample (~1 g) was transferred into YM broth (yeast extract 0.2%, malt extract 1.5%). These were incubated with horizontal shaking movement at three temperatures; 48C, 158C, and 258C for one week before being plated out onto YM agar, Medium 7, and Medium 4. For four weeks, all

plates were observed daily to sub-culture filamentous fungi. Sub-cultures were isolated on the same media as their parental plates. All isolates were sub-cultured until colonies of uniform physical appearance were obtained.

For more details, please see attached two files.

Hoping this will be helpful,

Rafik

with bacteria works extremely well. So it should give some help to fungi too

Sabrina Galdo Novo ithank you so much for your answer. in my case, i want to produce lignocellulytic enzyme

Rafik Karaman thank you for your answer. i am really appreciate your answer. i am using soil sample from antarctica and the sample need to be pretreated before isolate the fungi. after i pretreat the sample then it is enriched in cmc broth. now i am going to proceed with the next step which quite confusing