Does anyone have experience reviving the probiotic Lactobacillus reuteri from its freeze dried form?

31 May 2025 0 10K Report

I am having problem reviving freeze dried Lactobacillus reuteri (from an ampule) into MRS or M17 broth or agar. It grows very very weak on MRS agar but again when the tiny clear colonies are inoculated into MRS or M17 broth either in pH of 6.5 or 6 anaerobically or aerobically, no growth is observed. Any tips or solutions?

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