Does anyone have experience of detecting the copy number in transgenic plant, especially rice using qRT-PCR, as an alternate to southern blotting?

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Gustavo Acevedo-Hernandez

Maybe this can help. This review explains all the procedure: http://link.springer.com/article/10.1007%2Fs00299-004-0859-y

and here you can see it applied specifically to rice: http://link.springer.com/article/10.1007%2Fs00299-004-0881-0

Jo Vandesompele

Biogazelle offers copy number lab services, using either quantitative or digital PCR.

http://www.biogazelle.com/lab-services/workflows/other

Surender Khatodia

See this

Article Real time PCR based detection of transgene copy number in tr...

Allahbakhsh ..

Dear Rajaguru,

Thank for raising good question. There is a variation in literature to find exact copy number of your introduced gene (s) in transgenics. I would suggest you to go for the method that includes endogenous refernece gene as a control.